研究のタイプ: 医学／生物学の研究 (experimental study)

[GSM 1800MHzマイクロ波への慢性ばく露は培養海馬ニューロンにおける興奮性シナプス活性を低下させる] med./bio.

Chronic exposure to GSM 1800-MHz microwaves reduces excitatory synaptic activity in cultured hippocampal neurons

著者: Xu S, Ning W, Xu Z, Zhou S, Chiang H, Luo J
掲載誌: Neurosci Lett 2006; 398 (3): 253-257
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この研究は、免疫細胞化学と組み合わせた全細胞パッチクランプ分析を用いて、ラットの培養海馬ニューロンの機能に対するマイクロ波ばく露の影響を評価した。2.4 W / kgのSARで、1800-MHz GSMマイクロ波の慢性ばく露（8日間、1日15分）を細胞に与えた。その結果、ばく露により、α-アミノ-3-ヒドロキシ-5-メチル-4-ソキサゾールプロピオン酸（AMPA）型の微小興奮性シナプス後電位（mEPSC）の振幅の選択的減少が引き起こされた；その一方、AMPA mEPSCの周波数およびN-メチル-D-アスパラギン酸（NMDA）mEPSCの振幅は変化しなかった；さらに、GSMばく露により、培養ニューロンのシナプス後肥厚部（PSD）に最も豊富に存在するタンパク質（PSD95）の発現が減少した、と報告している。

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研究目的（著者による）

To assess the effects of chronic microwave exposure (GSM, 1800 MHz) on the activity of glutamate receptor channels in cultured hippocampal neurons of rats.

詳細情報

Glutamate receptor channels play a key role in excitatory synaptic transmission. Changes in glutamate receptor function may be involved in the mechanisms of the biological effects of GSM microwaves on brain activity.
One group of glutamate receptors are the ionotropic receptors. These are ligand gated ion channels. Two common glutamate receptors are the NMDA receptor (composed of the subunits NR1 and NR2, which again can have different variants, e.g. NR2A, NR2B) and the AMPA (alpha-amino-3-hydroxy-5-methyl-4-soxazole propionic acid) receptor (composed of the subunits GluR1, GluR2, GluR3, and GluR4) whose agonists include NMDA and AMPA.
In addition, the density of PSD95 was detected in exposed and sham exposed neurons. PSD95, a key component of the postsynaptic density (PSD), aggregates neurotransmitter receptors and downstream enzymes and thus ensures the selective activation of different signal transduction cascades within a single cell.
Cultures of hippocampal neurons were prepared from Sprague-Dawley rats.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1,800 MHz
Modulation type: pulsed
ばく露時間: repeated daily exposure, 15 min/day, for 8 days
  • SAR: 2.4 W/kg

ばく露1

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 repeated daily exposure, 15 min/day, for 8 days
Modulation
Modulation type pulsed
Duty cycle 12.5 %
Repetition frequency 217 Hz
Pulse type rectangular
ばく露装置
ばく露の発生源／構造
チャンバの詳細 Two rectangular waveguides, one for RF and one for sham exposure, were placed inside a conventional incubator at 37 °C, 5% CO2, and 95% humidity.
ばく露装置の詳細 Six 35-mm dishes were positioned in the H field maximum of the standing wave and exposed in E polarisation.
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 2.4 W/kg - 測定値および計算値 - -
測定および計算の詳細

The SAR was determined theoretically and confirmed experimentally.

Reference articles

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露中

研究の主なアウトカム（著者による）

Chronic exposure to GSM microwaves induced a decrease in the amplitudes of the miniature excitatory postsynaptic currents in AMPA receptors, whereas the amplitudes of NMDA receptors did not change. The frequency of the miniature excitatory postsynaptic currents of the AMPA receptors did not change under exposure.
Chronic exposure did not alter the density of the different receptor subunits.
Chronic microwave exposure induced a decrease in the density of PSD95.
The results indicated that GSM microwaves may reduce excitatory synaptic activity and the number of excitatory synapses in rat hippocampal neurons.

研究の種別:

研究助成

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