研究のタイプ: 医学／生物学の研究 (experimental study)

[超低周波磁界は熱による可逆的精巣損傷からの精子形成の回復を損なわせる] med./bio.

Extremely low-frequency magnetic fields can impair spermatogenesis recovery after reversible testicular damage induced by heat

著者: Tenorio BM, Ferreira Filho MB, Jimenez GC, de Morais RN, Peixoto CA, Nogueira Rde A, da Silva Junior VA
掲載誌: Electromagn Biol Med 2014; 33 (2): 139-146
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この研究は、ラットを用いて、熱ショックが引き起こした可逆的な精巣損傷からの精子形成の回復を60Hz、1mTの磁界ばく露が損なうか否かを調べた。36匹のWister雄の成獣ラット（120日齢）に単回の熱ショック（麻酔下で43℃の温水に12分間浸す）を与えた後、1回30分間、1日3回の磁界ばく露（または擬似ばく露）を15、30、60日間与えた（各群n=6）。その結果、熱ショック後の精子形成回復期における磁界ばく露は、精巣各部位の体積、細胞の超微細構造、組織形態的パラメータに変化を起こした；対照群では、熱ショック後60日で新しく構築された活発な精子形成を示したが、磁界ばく露群では同時点で広範な精巣組織の変性が残っていた；磁界は血漿中テストステロンを変化させなかった、と報告している。

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研究目的（著者による）

To investigate the effects of extremely low frequency magnetic fields on spermatogenesis recovery after testicular degeneration induced by heat in rats.

詳細情報

6 groups of rats were examined (n=6 per group). After a single testicular heat shock (43°C for 12 minutes), rats were exposed or sham exposed to the magnetic field for 15, 30, or 60 days.

ばく露

ばく露 パラメータ
ばく露1: 60 Hz
ばく露時間: three daily exposures of 30 minutes for 15, 30, or 60 days

ばく露1

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 three daily exposures of 30 minutes for 15, 30, or 60 days
ばく露装置
ばく露の発生源／構造
チャンバの詳細 rats were placed in a cylindrical compartment made of polyvinyl chloride with openings for air intake, centrally located in the Helmholtz coil
ばく露装置の詳細 pairs of Helmholtz coils; each coil was made of wire copper coated with vinyl, 154 turns, 38 cm diameter and 2.1 Ohm electrical impedance; coils were connected in parallel to a voltage generator, producing a uniform magnetic field; field lines crossed the animals transversely
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 1 mT - 測定値 - ± 0.1 mT
Additional parameter details

induced effective electromotive force on each testis was around 0.21 mV

Reference articles

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

Plasma testosterone concentration was not changed by the magnetic field exposure.
After 30 and 60 days of exposure, the weight of the testes was significantly decreased compared to the sham exposure, while the gonadosomatic index was only significantly decreased after 60 days of exposure.
After 15 days of exposure, the decrease in the volume of the seminiferous tubules lumen was the only significantly changed parameter in comparison to the sham exposure. However, after 30 and 60 days several changes regarding the volume of the testes components, the cell ultrastructure and histomorphometrical parameters occurred in the exposure group when compared to the testes of sham exposed rats. Summarized, rats from the sham exposure group had a reestablished and active spermatogenesis 60 days after the heat shock procedure, while exposed animals still showed serious testicular degeneration.
The authors conclude that extremely low frequency magnetic fields could inhibit the spermatogenesis recovery after testicular degeneration induced by heat in rats.

研究の種別:

研究助成

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