この研究の目的は、マウスの精巣組織に照射された送電線の電磁界((ELF)の推定される影響を評価することであった。40匹の成体BALB/cマウスが2つのグループに分けられた。1つのグループ(20匹)は1日24時間、毎日のばく露が行われ、他の擬似ばく露グループ(20匹)は実験対照として扱われた。7週間のばく露後、2つのグループのマウスが犠牲にされた。精巣組織は、血清の生化学的分析、組織病理学的検査、およびフローサイトメトリ-分析(FCM)によって評価された。電磁界ばく露は、DNA倍数性 (1n、2n、4n細胞)の分布にそれほど影響しなかったが、精巣の生殖細胞のS期分画および増殖指数を著しく減少させた。
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To investigate whether extremely low frequency magnetic fields similar to those of transmission lines affect the testis tissue of mice.
40 mice (20 male, 20 female) were divided into an exposure and a sham exposure group (10 males and 10 females in each group).
Remark EMF-Portal: The employment of female mice does not make sense in the examined endpoints (testis).
周波数 | 50 Hz |
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タイプ |
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ばく露時間 | continuous for 7 weeks (24 h/day) |
ばく露の発生源/構造 |
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チャンバの詳細 | 5 mice were kept in one cage, respectively |
ばく露装置の詳細 | electric field was induced by three-phase voltage lines which were connected to a secondary coil of the step-up transformer; lines were assumed to be straight, parallel to each other, with an equal distance (0.3 m); magnetic field was induced by three-phase current lines connected to a secondary coil of the step-down transformer and located beneath the insulating board; ground clearance of central line of the insulating board was 0.45 meter; primary coils of two transformers were respectively connected to two three-phase voltage dividers; mice cages were fixed at one side of B phase line (mid line) |
No significant differences in the serum level of gamma-glutamyltransferase were found between the exposure and the sham exposure group (remark EMF-Portal: no data given for the level of gamma-glutamyltransferase in the testis).
The histopathological examination of the testis revealed no differences between the exposure and the sham exposure group.
In exposed rats, the cell proliferation and the number of germ cells in the S-phase were significantly decreased compared to the sham exposed rats, while no difference regarding the number of chromosome sets was observed.
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