研究のタイプ: 医学／生物学の研究 (experimental study)

[ヒト末梢血リンパ球（hPBLs）に対する1.8 GHz無線周波放射の突然変異的および形態学的影響、ならびにイチョウ葉エキス（EGb 761）での前処理の防護的役割の可能性] med./bio.

Mutagenic and morphologic impacts of 1.8 GHz radiofrequency radiation on human peripheral blood lymphocytes (hPBLs) and possible protective role of pre-treatment with Ginkgo biloba (EGb 761)

著者: Esmekaya MA, Aytekin E, Ozgur E, Güler G, Ergun MA, Omeroglu S, Seyhan N
掲載誌: Sci Total Environ 2011; 410: 59-64
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この論文は、1.8GHz無線周波電磁界放射の単独ばく露および1.8GHz放射とイチョウ葉エキス（EGb 761）での前処理との組み合わせばく露によるヒト末梢血リンパ球（hPBLs）の突然変異的および形態学的影響を姉妹染色体分体交換および電子顕微鏡的観察で調べている。結論として、無線周波数電磁界放射はhPBLs における染色体損傷を誘導するが、この損傷はEGb 761での前処理によって軽減されるかも知れないと述べている。

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研究目的（著者による）

The effects of exposure of human peripheral blood lymphocytes to a 1.8 GHz electromagnetic field with or without co-exposure with a Ginkgo biloba extract on the mutation rate, cell viability and cell morphology should be investigated.

詳細情報

Cells were divided into 3 groups: 1) sham exposure, 2) exposure to the electromagnetic field, 3) exposure and addition of 100 µg/ml Ginkgo biloba extract "EGb 761" prior to the exposure.
All experiments were performed as triplicates.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1.8 GHz
Modulation type: pulsed
ばく露時間: continuous for 6, 8, 12 and 48 h
  • SAR: 0.21 W/kg mean

ばく露1

主たる特性
周波数 1.8 GHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 6, 8, 12 and 48 h
Modulation
Modulation type pulsed
Pulse width 0.576 ms
Duty cycle 12.5 %
Repetition frequency 217 Hz
ばく露装置
ばく露の発生源／構造
Distance between exposed object and exposure source 10 cm
チャンバの詳細 2 ml round bottom test tubes containing 0.5 lymphocytes solution
ばく露装置の詳細 rectangular horn antenna was placed vertically above the exposure tubes and radiating downwards; temperature inside the chamber was kept at 37°C by circulating water through a waterbed placed underneath the exposure tubes
Sham exposure A sham exposure was conducted.
Additional information no temperature difference was observed between sham exposed and exposed cells
パラメータ
測定量 値 種別 Method Mass 備考
SAR 0.21 W/kg mean 測定値および計算値 - -

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム（著者による）

At all points in time, the mutation rate was significantly higher in group 2 (only exposure) compared to the sham exposure group. At the same time, the mutation rate was significantly lower in group 3 (exposure + EGb 761) compared to group 2.
The cell viability was significantly reduced in group 2 compared to the sham exposure group at all points in time and it was significantly increased in group 3 after 8 hours and 24 hours of exposure compared to group 2.
Morphological abnormalities were spotted in group 2 at each point in time, with a tendency towards more pronounced and severe effects with growing exposure duration (remark EMF-Portal: no examination of other groups and no comparison; result unclear). The effects ranged from variations in the cell size after 6 hours of exposure to distinct organelle and membrane destruction after 48 hours of exposure.
The authors conclude that exposure of human peripheral blood lymphocytes to a 1.8 GHz electromagnetic field might increase the mutation rate, decrease cell viability and alter cell morphology, and that EGb 761 could reduce these effects.

研究の種別:

研究助成

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