研究のタイプ: 医学／生物学の研究 (experimental study)

[無線周波電磁界にインビトロで72時間ばく露されたヒト末梢血リンパ球における染色体の数的異常の上昇] med./bio.

Increased levels of numerical chromosome aberrations after in vitro exposure of human peripheral blood lymphocytes to radiofrequency electromagnetic fields for 72 hours

著者: Mazor R, Korenstein-Ilan A, Barbul A, Eshet Y, Shahadi A, Jerby E, Korenstein R
掲載誌: Radiat Res 2008; 169 (1): 28-37
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この研究は、ゲノムの不安定さに対する無線周波数電磁界（800MHzの連続波）の影響を、10人から採取したヒトリンパ球標本に72時間ばく露した場合について調べた。ばく露には特別設計した導波管共振器を用い、リンパ球は温度36-37度で、SAR２．９と４．１W/kgにばく露させた。分裂間期のFISH法により、1，10，11，17染色体にそれぞれ誘導される異数体を、ばく露レベル毎に測定した。この結果、1と10染色体では、高いSARで異数体が増加した一方、１１と１７染色体では低いSARで増加した：異数体増加の主体は多染色体性であった：温度の影響を33.5-40度について７２時間にわたって調べたが、37度の場合と差は無かった、などの所見を報告し、非熱的影響により異数体が増加するかも知れないと結論している。

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研究目的（著者による）

This study was designed to examine whether the exposure to 800 MHz electromagnetic field affects the number of chromosomes in human peripheral blood lymphocytes in vitro.

詳細情報

The human peripheral blood lymphocytes were obtained from 14 healthy male donors (23 to 39 years old). After exposure for 72 hours at two different specific absorption rates (2.9 and 4.1 W/kg) the chromosomes 1, 10, 11, and 17 were further investigated. Additionally, the effects of the temperature on the number of these chromosomes were determined in four samples incubated at 33.5, 37, 38.5, 40 °C.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 800 MHz
Modulation type: CW
ばく露時間: continuous for 72 h with a short break for handling every 24 h
  • SAR: 2.9 W/kg average over mass (1 g)
  • SAR: 4.1 W/kg average over mass (1 g)
  • SAR: 1 W/kg spatial average (1 - 8 W/kg calculated from experimental temperature measurements)

ばく露1

主たる特性
周波数 800 MHz
タイプ
  • electric field
ばく露時間 continuous for 72 h with a short break for handling every 24 h
Modulation
Modulation type CW
ばく露装置
ばく露の発生源／構造
ばく露装置の詳細 43 cm x 33 cm waveguide resonator
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 2.9 W/kg average over mass 計算値 1 g -
SAR 4.1 W/kg average over mass 計算値 1 g -
SAR 1 W/kg spatial average 計算値 - 1 - 8 W/kg calculated from experimental temperature measurements

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

Increased levels of aneuploidy depending on the chromosome studied as well as on the level of exposure were found. In chromosomes 1 and 10, there was increased aneuploidy at the higher specific absorption rate of 4.1 W/kg, while for chromosomes 11 and 17, the increases were observed only for the lower specific absorption rate of 2.9 W/kg.
No statistically significant effect of temperature on the level of aneuploidy was observed over the range of 33.5-40 °C compared to 37 °C.
These results suggest the possible existence of an athermal effect of radio frequency irradiation that causes increased levels of aneuploidy.

研究の種別:

研究助成

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