研究のタイプ: 医学／生物学の研究 (experimental study)

[マウス末梢リンパ球に対するGSM変調900MHz放射線へのイン・ビボばく露の影響] med./bio.

Effects of in vivo exposure to GSM-modulated 900 MHz radiation on mouse peripheral lymphocytes

著者: Gatta L, Pinto R, Ubaldi V, Pace L, Galloni P, Lovisolo GA, Marino C, Pioli C
掲載誌: Radiat Res 2003; 160 (5): 600-605
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この研究は、900 MHz GSM変調電磁界への毎日の全身ばく露が、C57BL/6マウスの脾臓リンパ球に影響を与えるか否かを調べた。マウスは、TEMセル内で、SARが1または2 W/ kgの電磁界ばく露を、2時間／日で、1、2または4週間、受けた。無処置群、擬似ばく露群も実験に含めた。全ての動物は、ばく露（擬似ばく露）終了後に屠殺し、脾臓細胞を採取した。その結果、脾臓細胞の数、B細胞とT細胞の割合、T細胞亜集団（CD4とCD8）の分布は、ばく露によって変化しなかった；採取されたTおよびB細胞を、ex vivoで、特定のモノクローナル抗体またはLPSで刺激し、細胞増殖、サイトカイン産生、活性化マーカーの発現の誘導を観察したが、Tリンパ球またはBリンパ球のどちらにおいても、ばく露群と擬似ばく露群の間に大きな違いは見られなかった；1または2 W / kgへの1週間のばく露後、IFN-γ（インターフェロンγ）産生の増加が観察されたが、2または4週間のばく露後には見られなかった；これは、他のストレス因子と同様に、免疫系がRF放射に適応した可能性があることを示唆する、と報告している。

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研究目的（著者による）

To evaluate whether daily whole-body exposure of mice to 900 MHz GSM-modulated irradiation could affect spleen lymphocytes. T cells and B cells were stimulated ex vivo using specific monoclonal antibodies or lipopolysaccharides to induce cell proliferation, cytokine production and expression of activation markers.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
Modulation type: pulsed
ばく露時間: repeated daily exposure, 2 h/day for 1, 2 or 4 weeks
  • SAR: 1 W/kg mean
  • SAR: 2 W/kg mean

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 repeated daily exposure, 2 h/day for 1, 2 or 4 weeks
Modulation
Modulation type pulsed
Additional information

basic GSM modulation
ばく露装置
ばく露の発生源／構造
  • セルラ－電話
  • TEMセル
  • 12 x 12 x 120 cm
ばく露装置の詳細 Mice were housed individually in transparent Perspex jigs with openings to provide air circulation. Four mice were placed in the cell (two above and two below the septum with daily clockwise rotation) with their caudal axis parallel to the direction of propagation.
Additional information A blinded procedure was used for exposure. Sham-exposed mice experienced the same conditions except for the EMF. A control group was kept with minimal handling. A water cooling system was set up with two external metal jackets filled with circulating water fed through a thermostatic bath (20°C) and placed in contact with the bottom walls. The maximum difference between exposed and sham-exposed groups was < 0,5°C.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 1 W/kg mean 測定値および計算値 - -
SAR 2 W/kg mean 測定値および計算値 - -
Additional parameter details

Average efficiency for live mice was 0.38 ± 0.09 W/kg/Win.

測定および計算の詳細

The amplified signal was controlled by power meters via bi-directional couplers. The EMF distribution in the cell, with and without objects inside, was assessed using the numerical code HFSS (High Frequency Structure Simulator) and by measurements with network analyser and calibrated E-field probes. Numerical dosimetry was performed using two codes (XFDTD and CST Microwave Studio). Mice were assumed to be cylindrical and homogeneous and weigh 24 g. Experimental dosimetry was done using the power balance method with both homogeneous phantoms and live mice. The total uncertainty was evaluated to be about 20% at 95% confidence.

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

The number of spleen cells, the percentages of B cells and T cells, and the distribution of T cell subpopulations were not changed by irradiation. The findings did not show relevant differences in either T lymphocytes or B lymphocytes from animals exposed to an SAR of 1 or 2 W/kg and sham-exposed animals with few exceptions. After 1 week of exposure to 1 or 2 W/kg, an increase in IFN-gamma production was revealed that was not evident when the irradiation was prolonged to 2 or 4 weeks. This indicates that the immune system might have adapted to radiofrequency irradiation as it does with other stressing agents.
In conclusion, the in vivo data indicate that the T cell and B cell compartments were not substantially affected by radiofrequency irradiation and that a clinically relevant effect of the irradiation on the immune system is unlikely to occur.

研究の種別:

研究助成

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