[マウス骨髄細胞の造血系細胞への分化能に対するGSM変調900MHz無線周波電磁界の影響] med./bio.

The ability of bone marrow precursor cells from mice, exposed to a radio frequency field, to colonize thymus and spleen of lethally x-irradiated mice and to differentiate should be investigated. The study is an expansion to the precursor study of Prisco et al., 2008

The study should verify the results of the precursor study and check them with the help of a new, sensitive method. Therefore, two different approaches were conducted: In approach 1, lethally x-irradiated wild type B/6 mice received injections with bone marrow cells from syngenic exposed (1a, n=8), sham exposed (1b, n=8) and cage control animals (1c, n=8). In approach 2, three X-irradiated Rag2 deficient mice received 50:50 mixtures of bone marrow cells: 2a) from cage control (wild type B/6) and sham exposure (congenic B/6)), 2b) from sham exposure (congenic B/6) and exposure (wild type B/6) and 2c) from cage control (congenic B/6) and exposure (wild type B/6).
As Rag2 deficient mice cannot produce mature B cells and T cells, approach 2 was used to detect differences between two different treatments, respectively, with a very high sensitivity, as those cells develop from the transplanted bone marrow cells, compete in the colonization of thymus and spleen and their origin can be tracked. Wild type and congenic donor mice express different variants of the Thy1 surface molecule CD90 on T cells (Thy1^a und Thy1^b) and of the immunoglobulin heavy chain Igh on B cells (Igh^a und Igh^b), which can be detected with specific antibodies. Investigation took place 12 weeks after transplantation.

• 動物
• mouse/C57BL/6Thy1^bIgh^b (wild type B/6)
• 全身

No differences regarding total thymocyte and spleen cell numbers, percentages of subpopulations, cell proliferation and level of interferon-gamma could be found between the groups of test approach 1. Approach 2 revealed a slight but insignificant higher amount of cells stemmed from the cage control animals in mice 2a) and 2c). This was interpreted as a decreased fitness of cells from sham exposure and exposure animals due to the increased stress they had encountered during the exposure procedure.
The authors conclude that their results indicate no effects of a chronic whole body exposure to a 900 MHz GSM electromagnetic field on the ability of bone marrow precursor cells from mice to colonize thymus and spleen and to differentiate and thus that the results of their precursor study are verified.