研究のタイプ: 医学／生物学の研究 (experimental study)

[In vitroで活性化させたヒト血液免疫細胞に対する900 MHzマイクロ波GSM信号の免疫影響] med./bio.

Immunotropic influence of 900 MHz microwave GSM signal on human blood immune cells activated in vitro

著者: Stankiewicz W, Dabrowski MP, Kubacki R, Sobiczewska E, Szmigielski S
掲載誌: Electromagn Biol Med 2006; 25 (1): 45-51
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この研究は、先行研究（低レベル（SAR = 0.18 W / kg）のパルス変調1300 MHzマイクロ波ばく露を受け、その後培養されたG（0）期の末梢血単核細胞（PBMC）での免疫活性変化を報告（Dabrowski et al. 2003））を背景に、細胞周期（G（1）、S）の活動期の培養免疫細胞にマイクロ波ばく露を与えて、電磁界に対する反応が見られるか否かを調べた。インキュベータ内に置かれた無反射チャンバ内で、培養細胞を入れたマイクロプレートに900 MHzのGSM模擬信号（27 V / m）をSAR 値0.024 W / kgで与えた。その結果、ばく露群では、対照群に比べ有意に、マイトジェンに対する応答が高く、単球の免疫原性活性（LM指数）が高かった；LM指数（Dabrowski et al. 2001）は、リンパ球の細胞分裂反応に対するモノカインの影響を表す、と報告している。

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研究目的（著者による）

The authors investigated how active cells, e.g. stimulated in vitro with mitogens and entering G1 phase and S phase, will react to the exposure to microwaves.

詳細情報

Cells were stimulated with phytohemagglutinin or with concanavalin A.
Cells were exposed immediately after entering the G1 phase (first day of exposure), again when the majority of cells responding to mitogen entered the S phase (second day of exposure), and finally when the responding cells reached G2 phase and mitosis (third day of exposure).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
Modulation type: pulsed
ばく露時間: repeated daily exposure, 15 min at the beginning of each day, for 3 days

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
特性
  • near field
ばく露時間 repeated daily exposure, 15 min at the beginning of each day, for 3 days
Modulation
Modulation type pulsed
Pulse width 577 µs
Repetition frequency 217 Hz
Additional information

GSM system
ばく露装置
ばく露の発生源／構造
  • セルラ－電話
  • 無響室
  • fed from an external generator
Distance between exposed object and exposure source 6 cm
チャンバの詳細 A miniature anechoic chamber of 23 x 23 x 23 cm internal dimensions was located inside an incubator.
ばく露装置の詳細 A microplate with cultured cells was placed in the middle of the chamber with a mobile phone on the floor.
パラメータ
測定量 値 種別 Method Mass 備考
電界強度 27 V/m - 測定値 - ±15%
電力密度 1.93 W/m² - 測定値 - -
SAR 0.024 W/kg - 測定値および計算値 - -
測定および計算の詳細

The electric field distribution was measured using a meter and a probe containing a dipole-diode detector to find the minimum distance where the field was sufficiently homogeneous (±15%). SAR was determined for both components of the EF separately by measuring samples in a waveguide for two polarisations [Dabrowski et al.. 2003].

Reference articles

  • Dabrowski MP et al. (2003): [低レベル1300 MHzパルス変調マイクロ波へあらかじめばく露されたヒト単核球細胞での免疫効果]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

Peripheral blood mononuclear cells exposed to microwaves demonstrated significantly higher response to mitogens and higher immunogenic activity of monocytes than control cultures. The proliferative response of T lymphocytes exposed to microwaves increased significantly.
The data suggest that immune activity of responding lymphocytes and monocytes can be additionally intensified by 900 MHz microwaves.

研究の種別:

研究助成

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