The exposure chamber design was principally the same as described before [Pakhomov et al., 2000]. The chamber, provided with a continuous but laminar flow of fresh artificial cerebrospinal fluid (ACSF) of about 20 mm depth, was mounted on top of a waveguide opening sealed with a sapphire matching plate covered with 0.5 mm Plexiglas, which served as a chamber bottom.
Aufbau
A brain slice was centered in a small frame (also made of 0.5 mm Plexiglas), which was fixed to the chamber bottom and aligned with the waveguide axis. MW exposures were performed essentially the same way as described before [Pakhomov et al., 2002].
Pakhomov AG et al.
(2002):
Comparison of dose dependences for bioeffects of continuous-wave and high-peak power microwave emissions using gel-suspended cell cultures
Pakhomov AG et al.
(2000):
Comparative effects of extremely high power microwave pulses and a brief CW irradiation on pacemaker function in isolated frog heart slices
Air Force Office of Scientific Research (AFOSR), USA
U.S. Medical Research and Materiel Command (USAMRMC)
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