The waveguide exposure chamber was an aluminium RF resonator with a plastic cultivation chamber and a water circulation heat exchanger for keeping the temperature of the cell culture medium constant (± 0.3°C). The water circulated under a glass surface on which the cell-culture dishes were placed. Glass Petri dishes were used instead of plastic dishes for better thermal contact. There were two identical chambers, one for exposure and one for sham-exposure. However, the temperature of the water circulation heat exchanger in the exposure chamber (37.5 ± 0.3°C) was found to be, on average, 0.8°C higher than in the sham exposure chamber (36.7 ± 0.3°C), despite the temperature control. Warm air (approx. 37°C) and CO2 (5%) were fed into the cultivation chambers from a cell culture incubator.
Aufbau
The RF power source consisted of a signal generator, a 217 HzGSM modulator, and power amplifier. RF power was fed into the exposure chamber with a monopole post, and the electric field inside the chamber, below the heat exchanger, was monitored from another monopole post with an RF power meter.
Zusatzinfo
Two Petri dishes were exposed simultaneously, and two dishes were sham-exposed. This was repeated 3-5 times at each exposure level and duration. In separate additional experiments, cells were incubated in ordinary cell culture incubators for 2 h at 37.6°C and 36.5°C measured with a Fluke thermometer and thermocouple in the medium. Magnetic fields were measured in both incubators to be 1.1 µT at 50 Hz at the position of the cell dishes. Cells were handled exactly in the same way as in the exposures in the waveguide system.
Billaudel B et al.
(2009):
Effects of exposure to DAMPS and GSM signals on ornithine decarboxylase (ODC) activity: II. SH-SY5Y human neuroblastoma cells
Billaudel B et al.
(2009):
Effects of exposure to DAMPS and GSM signals on ornithine decarboxylase (ODC) activity: I. L-929 mouse fibroblasts
Höytö A et al.
(2007):
Ornithine decarboxylase activity is affected in primary astrocytes but not in secondary cell lines exposed to 872 MHz RF radiation
Höytö A et al.
(2007):
Ornithine decarboxylase activity of L929 cells after exposure to continuous wave or 50 Hz modulated radiofrequency radiation--a replication study
Desta AB et al.
(2003):
Non-thermal exposure to radiofrequency energy from digital wireless phones does not affect ornithine decarboxylase activity in L929 cells
Litovitz TA et al.
(1997):
Bioeffects induced by exposure to microwaves are mitigated by superposition of ELF noise
Penafiel LM et al.
(1997):
Role of modulation on the effect of microwaves on ornithine decarboxylase activity in L929 cells
Litovitz TA et al.
(1993):
The role of coherence time in the effect of microwaves on ornithine decarboxylase activity
Byus CV et al.
(1988):
Increased ornithine decarboxylase activity in cultured cells exposed to low energy modulated microwave fields and phorbol ester tumor promoters
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