研究のタイプ: 医学／生物学の研究 (experimental study)

[1800 MHz無線周波放射へのばく露は胚性神経幹細胞の神経突起伸長を傷害する] med./bio.

Exposure to 1800 MHz radiofrequency radiation impairs neurite outgrowth of embryonic neural stem cells

著者: Chen C, Ma Q, Liu C, Deng P, Zhu G, Zhang L, He M, Lu Y, Duan W, Pei L, Li M, Yu Z, Zhou Z
掲載誌: Sci Rep 2014; 4: 5103
RISフォーマットで参考文献をダウンロード

この研究は、受精後13.5日のマウス終脳から採取し、培養した胚性神経幹細胞（eNSCs）に対する1800 MHz無線周波放射（RF-EMF）のばく露の影響を調べた。ばく露条件は、SAR1、2、4 W/kg、ばく露期間1、2、3日間とした。その結果、eNSCのアポトーシス、増殖、細胞周期、関連遺伝子のmRNA 発現にはRF-EMFばく露の影響は見られなかった；また、星状膠細胞の分布比も変化しなかった；しかし、4 W/kgの3日間ばく露群で、神経突起伸長が阻害された、と報告している。

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研究目的（著者による）

The effects of an in vitro exposure to a 1800 MHz electromagnetic GSM field on the differentiation of mouse embryonic neural stem cells should be investigated.

詳細情報

All-trans-retinoic acid was used as positive control in the testing of neurite outgrowth because of its promotive effects on neuronal cell differentiation. PD98059 was used as negative control in these tests as it is a specific inhibitor of the ERK1/2 pathway, which regulates neurite outgrowth.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1,800 MHz
ばく露時間: intermittent for 1 to 3 days (5 min field on, 10 min field off)
  • SAR: 1 W/kg mean
ばく露2: 1,800 MHz
ばく露時間: intermittent for 1 to 3 days (5 min field on, 10 min field off)
  • SAR: 2 W/kg mean
ばく露3: 1,800 MHz
ばく露時間: intermittent for 1 to 3 days (5 min field on, 10 min field off)
  • SAR: 4 W/kg mean

ばく露1

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
ばく露時間 intermittent for 1 to 3 days (5 min field on, 10 min field off)
ばく露装置
ばく露の発生源／構造
  • sXc-1800 exposure system (GSM simulation)
チャンバの詳細 two identical chambers (exposure and sham exposure) mounted inside the same cell culture incubator; temperature was maintained at 37°C ± 0.5°C with max. variance between the chambers of 0.3°C
ばく露装置の詳細 electromagnetic field was modulated to simulate GSM Talk-signal mode
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 1 W/kg mean - - -

ばく露2

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
ばく露時間 intermittent for 1 to 3 days (5 min field on, 10 min field off)
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 2 W/kg mean - - -

ばく露3

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
ばく露時間 intermittent for 1 to 3 days (5 min field on, 10 min field off)
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 4 W/kg mean - - -

Reference articles

  • Franzellitti S et al. (2010): [高周波電磁界（GSM 1.8 GHz）から生じるヒト栄養膜細胞株HTR-8/SVNeoにおける過渡的DNA損傷のアルカリ・コメットアッセイでの評価]
  • Schuderer J et al. (2004): [1800 MHzのインビトロ実験に用いる強力なばく露および環境制御機能を備えた高尖頭SARばく露装置]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム（著者による）

No significant differences could be observed for apoptosis, proliferation, cell cycle analysis and the ratio of differentiated astrocytes and neurons between exposure and sham exposure. However, cells exposed to the 4 W/kg field for 3 days showed a significantly decreased neurite outgrowth compared to sham exposure. Simultaneously, RNA and protein expression of the pro-neuronal genes Tuj1, Ngn1 and NeuroD were significantly decreased while Hes1 expression, an inhibitor of pro-neuronal genes, expression was significantly increased.
The authors conclude that an in vitro exposure of mouse embryonic neural stem cells to a 1800 MHz electromagnetic GSM field impairs the neurite outgrowth in differentiated neurons and that further studies should investigate possible consequences.

研究の種別:

研究助成

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