研究のタイプ: 医学／生物学の研究 (experimental study)

[マウスの小核を有する多染性赤血球に対する50 Hz磁界長期ばく露の影響] med./bio.

Effect of long-term 50 Hz magnetic field exposure on the micronucleated polychromatic erythrocytes of mice

著者: Alcaraz M, Olmos E, Alcaraz-Saura M, Achel DG, Castillo J
掲載誌: Electromagn Biol Med 2014; 33 (1): 51-57
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この研究は、50Hzの超低周波磁界（ELF-EMF）の長期ばく露（7、14、21、28日）によるマウスでの遺伝毒性的影響を分析するため、マウス骨髄の小核を有する多染性赤血球（MNPCEs）の出現頻度を測定し、50 cGy のX線による誘発される出現頻度と比較した。磁界強度はコイルの中心で200±20 μT、マウスはそのゾーンに7、14、21、28日滞在した。また、放射線防護力を高める4種の抗酸化剤の投与によりこの染色体損傷が減弱されるか否かを調べた。この実験では、磁界については、4種の薬剤を投与後、28日のばく露を行い、X線については、薬剤投与の5日前と1日後の照射の2通りを行った。その結果、ELF-EMF、X線の処置群では、対照群に比べ小核を有する細胞が増加した；抗酸化剤によるゲノム保護作用は電離放射線による損傷に対してのみ見られた、と報告している。

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研究目的（著者による）

To investigate the possible genotoxic effect of extremely low frequency magnetic fields in mouse bone marrow.

詳細情報

The results of the magnetic field exposure were compared to the effects of X-radiation (50 cGy).
In total, 168 mice were used. For the magnetic field exposures as well as for the X-radiation, 14 groups were examined, resepctively (n=6 per group).
In the first experiment, mice were exposed to the magnetic field for 7, 14, 21 or 28 days and killed one day after the last exposure. The control group without magnetic field exposure was kept for 28 days. Four antioxidants (dimethyl sulfoxide (DMSO), 6-n-propyt-2-thiouracil (PTU), grape procyanidins (P), citrus flavonoids extract (CE)) were administered to try to reduce the chromosomal damage. These substances were given for 28 days. Furthermore, control groups without exposure which received the substances for 28 days were examined.
In a second experiment, mice were treated with X-radiation. The same antioxidants as in the magnetic field exposure groups were administered 5 days before or 1 day after the radiation. Additional control groups without X-radiation but with the antioxidants were examined.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 7, 14, 21 or 28 days

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 7, 14, 21 or 28 days
ばく露装置
ばく露の発生源／構造
ばく露装置の詳細 experimental device consisted of three 100-tum coils of 1 mm diameter copper wire; to feed the coils, a variable intensity source of 50 Hz was constructed, electric current was 700 mA
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 200 µT - 測定値 - ± 20 µT
測定および計算の詳細

field was measured every 12 hours

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

The bone marrow of magnetic field exposed mice (p value < 0.01) and of X-radiated mice (p value < 0.001) showed a significantly increased number of micronucleated erythrocytes in comparison to the corresponding control group. In the magnetic field exposed groups, an administration of the antioxidants did not significantly influence the number of micronuclei. However, groups that were treated with X-radiation and antioxidants had significantly decreased numbers of micronuclei compared to X-radiation alone.
The authors conclude that the extremely low frequency magnetic field had a genotoxic effect in mouse bone marrow and that the administered antioxidants could not prevent this effect.

研究の種別:

研究助成

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