この研究は、塩化鉄（III）（FeCl3）の添加あり、または添加なしの大腸菌培養に超低周波（ELF）磁界ばく露を与え、細菌の増殖と形態に及ぼす影響を調べた。ELF磁界ばく露には、50Hz正弦波磁界を発生するヘルムホルツコイル対を用いた。磁界は、コイル対の軸に沿ってほぼ一様であった。異なる濃度のFeCl3添加または無添加の細菌試料に、50 Hz、2 mTの磁界ばく露を24時間与えた。細胞生存率は、標準的なプレートカウント法により、生存可能コロニー数（1mlあたりのコロニー形成単位）で評価した。走査型電子顕微鏡を用いて、大腸菌の表面形態への影響を調べた。その結果、ELFばく露群と擬似ばく露群の間に、細胞生存率の有意な差はなかった；同様に、FeCl3添加の有無は、細胞生存率を変化させなかった；ただし、ELFばく露群では、大腸菌の細胞表面にいくつかの形態学的変化が観察され、細孔の形成と膜の破壊が見られた、と報告している。
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To examine the effects of 50 Hz magnetic fields alone or in combination with FeCl3 on the cell viability and morphology of Escherichia coli.
Escherichia coli cells were grown in culture medium without or with different concentrations of iron(III) chloride (0.2, 2 and 20 g/l FeCl3) while being exposed or sham exposed.
| 周波数 | 50 Hz |
|---|---|
| タイプ |
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| 波形 |
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| ばく露時間 | continuous for 24 hours |
| ばく露の発生源／構造 | |
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| チャンバの詳細 | 30 ml plastic culture flasks containing E. coli in a fluid medium were used |
| ばく露装置の詳細 | coils were made up of 154 turns of copper wire with a diameter of 2.2 mm having inner and outer diameters of 40.5 and 45.5 cm, respectively; coils were placed in horizontal plane to produce vertically oriented magnetic field; distance between the coils was 21.375 cm; electrical parameters of the coils were resistance of 1.2 ohm and inductance of 19.6 mH; coils were driven with a variable transformer with a power of 2.7 kVA; system was mounted on a wooden frame; culture flasks were placed at the center of the coil-based exposure system (homogenous exposure region) in vertical position, so that the magnetic field was perpendicular to the bottom of the flasks |
| Sham exposure | A sham exposure was conducted. |
No significant differences between exposed and sham exposed cells were observed regarding cell viability. Furthermore, no significant influence of FeCl3 supplementation of the culture medium on the cell viability was found.
While the morphology of sham exposed cells appeared normal in the scanning microscopy, exposed cells showed structural damages such as various pores with different sizes on the cell membrane.
The authors conclude that 50 Hz magnetic fields do not influence the cell viability of Escherichia coli but could affect the surface morphology.
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