研究のタイプ: 医学／生物学の研究 (experimental study)

[マイクロ波と細胞性免疫：その１　マウス細胞のでTNF産出に対する全身マイクロ波放射の影響] med./bio.

Microwaves and cellular immunity. I. Effect of whole body microwave irradiation on tumor necrosis factor production in mouse cells

著者: Fesenko EE, Makar VR, Novoselova EG, Sadovnikov VB
掲載誌: Bioelectrochem Bioenerg 1999; 49 (1): 29-35
RISフォーマットで参考文献をダウンロード

1μW/cm2電力密度で8.15-18 GHzでオスのNMRIマウスを全身マイクロ波放射すると、腹膜のマクロファージと脾臓Tリンパ球にTNF産出の大きな増加をもたらされた。マイクロ波放射はT細胞に影響を与え、細胞分裂刺激への反応を促進するように働く。細胞性免疫の刺激に必要なばく露期間は5時間から3日である。7日間の慢性的にばく露させたマウスは、腹膜のマクロファージにおいてTNF生産を減少させた。24時間ばく露では、TNF生産と免疫反応を減少させた。ばく露停止3日後もこの症状は続いた。マイクロ波の処置はサイトカイン合成の刺激の一つであるリポ多糖体と比べると、TNFはより増えた。細胞免疫プロセスを干渉する要因としてのマイクロ波の役割を述べている。

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研究目的（著者による）

To study the effect of weak 8.15-18 GHz microwaves on the tumor necrosis factor secretion and the T lymphocytes proliferative response.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 10 GHz
Modulation type: CW
ばく露時間: continuous for 0.5 h to 7 days
  • 電力密度: 1 µW/cm² spatial average (0.2-1.6 µW/cm²)
  • SAR: 5 mW/kg (2-5 mW/kg)
ばく露2: 8.15–18 GHz
ばく露時間: continuous for 0.5 h to 7 days
  • 電力密度: 1 µW/cm² spatial average (0.2-1.6 µW/cm²)
  • SAR: 5 mW/kg (2-5 mW/kg)

General information

A total of 45 and 60 animals were exposed in E1 and E2, respectively. The same numbers of animals were used for cage control. In both experiments, 10 animals were sham irradiated and 10 were untreated. The procedure was double blind.

ばく露1

主たる特性
周波数 10 GHz
タイプ
  • electromagnetic field
波形
  • sinusoidal
ばく露時間 continuous for 0.5 h to 7 days
Modulation
Modulation type CW
ばく露装置
ばく露の発生源／構造
Distance between exposed object and exposure source 80 cm
ばく露装置の詳細 Groups of five unrestrained mice were exposed in plastic cages (25 cm x 25 cm x 45 cm) without roof. There was no reflecting material on the floor beneath the animals.
Sham exposure A sham exposure was conducted.
Additional information Unexposed cage control animals were housed in a separate room under the same conditions.
パラメータ
測定量 値 種別 Method Mass 備考
電力密度 1 µW/cm² spatial average 測定値 - 0.2-1.6 µW/cm²
SAR 5 mW/kg - 推定値 - 2-5 mW/kg
測定および計算の詳細

The bell-shaped field distribution in the animal location was mapped using an incident power density meter and a thermistor type probe of 15 cm diameter. Since it was very difficult to perform experimental measurements of real SAR [Khizhnyak et al., 1994], the range was estimated assuming a penetration depth of 2-5 mm [Furia et al., 1986].

ばく露2

主たる特性
周波数 8.15–18 GHz
タイプ
  • electromagnetic field
波形
  • sinusoidal
ばく露時間 continuous for 0.5 h to 7 days
Modulation
Modulation type cf. additional information
Additional information

swept frequency from 8.15 GHz to 18 GHz with 1 s direct and 16 ms reversed time (output stability better than 12 dB)
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
電力密度 1 µW/cm² spatial average 測定値 - 0.2-1.6 µW/cm²
SAR 5 mW/kg - 推定値 - 2-5 mW/kg

Reference articles

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

Whole body microwave irradiation caused a significant enhancement of tumor necrosis factor production in peritoneal macrophages and splenic T lymphocytes. Microwave exposure affected T cells, facilitating their capacity to proliferate in response to mitogenic stimulation. The irradiation duration necessary for the stimulation of cellular immunity ranged from 5 h to 3 days.
Chronic exposure for 7 days produced the decreasing of tumor necrosis factor production in macrophages.
The irradiation of the animals for 24 h increased the tumor necrosis factor production and immune proliferative response, and these stimulatory effects persisted over 3 days after the termination of exposure.
Microwave treatment increased the endogenously produced tumor necrosis factor more effectively than did lipopolysaccharide (one of the most potential stimuli of synthesis of this cytokine).

研究の種別:

研究助成

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