研究のタイプ: 医学／生物学の研究 (experimental study)

[細胞傷害性Tリンパ球に対する等温条件での無線周波電磁界の影響] med./bio.

Effect of isothermal radiofrequency radiation on cytolytic T lymphocytes

著者: Cleary SF, Du Z, Cao G, Liu LM, McCrady C
掲載誌: FASEB J 1996; 10 (8): 913-919
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この研究は、細胞傷害性Tリンパ球（CTLL-2）におけるインターロイキン2（IL-2）依存性の増殖に対する無線周波（RF：2450MHz）電磁界ばく露の影響の有無を調べた。様々な生理学的濃度のIL-2 細胞の存在下（あるいは存在なし）で培養したCTLL-2にRFばく露を行い、ばく露終了の直後および24時間後の三重水素チミジン取り込み測定により増殖への影響を調べた。その結果、SARが25W/kg（誘導電界強度98.4V/m）以上で、特にIL-2が低濃度の場合、CTLL-2の増殖が有意に低下した；それより低いSARでは、ばく露直後の増殖は上昇したが、24時間後では低下した､と報告している。

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研究目的（著者による）

To study the effects of radiofrequency irradiation on interleukin-2-dependent proliferation of cytolytic T lymphocytes (CTLL-2).

詳細情報

After antigenic activation CTLL-2 express high-affinity receptors for the hormone interleukin-2. Binding of interleukin-2 is necessary and sufficient for T cell proliferation. After exposure to radiofrequency cells were cultured at various physiological concentrations of interleukin-2 (0, 0.62, 1.25, 2.5, 5, 10, 20, or 40 U/ml).
CTLL-2 cells were also incubated for 2 h at 37, 38, 39, 40, or 41°C without electromagnetic field exposure (temperature control).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 2.45 GHz
Modulation type: CW
ばく露時間: continuous for 2 h
ばく露2: 2.45 GHz
Modulation type: pulsed
ばく露時間: continuous for 2 h

ばく露1

主たる特性
周波数 2.45 GHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 2 h
Modulation
Modulation type CW
ばく露装置
ばく露の発生源／構造
ばく露装置の詳細 Cells were simultaneously RF and sham exposed in identical waveguide exposure chambers maintaining isothermal conditions at 37 ± 0.1°C as described previously [Liu et al., 1988].
Sham exposure A sham exposure was conducted.
Additional information Thermal control cultures were incubated for 2 h at 37, 38, 39, 40, or 41°C in the chambers used for MW exposure.
パラメータ
測定量 値 種別 Method Mass 備考
電界強度 139 V/m - 測定値 - -
SAR 50 W/kg - 測定値 - -
電界強度 98.4 V/m - 測定値 - -
SAR 25 W/kg - 測定値 - -
電界強度 44 V/m - 測定値 - -
SAR 5 W/kg - 測定値 - -
測定および計算の詳細

SAR measurements, intrasample temperature and SAR distributions, and temperature control procedures were previously described [Liu et al., 1988].

ばく露2

主たる特性
周波数 2.45 GHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 2 h
Modulation
Modulation type pulsed
Pulse width 6.67 ms
Repetition frequency 50 Hz
Additional information

simulating PCS modulation
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
電界強度 76.2 V/m peak 測定値 - -
SAR 15 W/kg peak 測定値 - -
電界強度 44 V/m average over time 測定値 - -
SAR 5 W/kg average over time 測定値 - -

Reference articles

  • Liu LM et al. (1988): [相転移温度でのリポソームの透過性に対する2.45 GHzマイクロ波および100 MHz無線周波放射の影響]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

Exposure to 2450 MHz radiofrequency irradiation at specific absorption rates of greater than 25 W/kg induced a consistent, statistically significant reduction in CTLL-2 proliferation, especially at low interleukin-2 concentrations. At lower specific absorption rates, exposure increased CTLL-2 proliferation immediately after exposure but reduced 24 h postexposure proliferation.
Radiofrequency irradiation effects depended on the mitotic state of the cells at the time of exposure. Comparison of the effects of temperature elevation (temperature control) and radiofrequency irradiation indicated significant qualitative and quantitative differences.

研究の種別:

研究助成

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