この研究は、間葉系幹細胞（MSCs）のドーパミン作動性（DA）ニューロンへの分化に対する電磁界ばく露（50 Hz；強度は40または400µT；１日１時間のばく露を１週間）の影響を調べるため、これら各条件のばく露を受けたMSCsをそれぞれパーキンソン病ラットモデルの左脳室に注入し、この2週間後にラットを検査した。その結果、（著者の記述によれば）注入された細胞はDAニューロンに分化し、黒質緻密部に散発的に定着した；このような移植を受けたラットでは対照群に比べパーキンソン症状の低下効果が見られたと報告している。
The detailed summary of this article is not available in your language or incomplete. Would you like to see a complete translation of the summary? Then please contact us →
The effects of exposure of rat mesenchymal stem cells to a 50 Hz magnetic field on cell differentiation and behavioral and histological changes in Parkinson's disease model rats after implantation of these stem cells into the brain.
Parkinson's disease was experimentally induced in rats via injections of 6-hydroxy-dopamine into left substantia nigra of the brain, which caused the loss of dopaminergic neurons.
Rats were divided into the following groups (n=8, respectively): 1) injections of saline solution into the brain (control group), 2) injection of saline solution with ascorbic acid (vehicle of 6-hydroxy-dopamine), 3) injection of 4 µg 6-hydroxy-dopamine, 4) injection of 6-hydroxy-dopamine and stem cells that were exposed to a 40 µT magnetic field for a week, 5) injection of 6-hydroxy-dopamine and stem cells that were exposed to a 400 µT magnetic field for a week, 6) injection of 6-hydroxy-dopamine and sham exposed stem cells.
(remark EMF-Portal: Though stated differently in the abstract, rats were probably killed 4 weeks after injections.)
| 周波数 | 50 Hz |
|---|---|
| タイプ |
|
| ばく露時間 | continuous for 1 hour/day for 1 week |
| ばく露の発生源／構造 | |
|---|---|
| チャンバの詳細 | culture flasks |
| ばく露装置の詳細 | horizontal Helmholtz coil (300 turns, distance of 6 cm and internal diameter of 16 cm) embedded in an open plexiglass rectangular frame and placed in an incubator (5% CO2, 37 °C) |
| Sham exposure | A sham exposure was conducted. |
| 測定量 | 値 | 種別 | Method | Mass | 備考 |
|---|---|---|---|---|---|
| 磁束密度 | 40 µT | - | 測定値 | - | - |
| 周波数 | 50 Hz |
|---|---|
| タイプ |
|
| ばく露時間 | continuous for 1 hour/day for 1 week |
| ばく露の発生源／構造 |
|
|---|
| 測定量 | 値 | 種別 | Method | Mass | 備考 |
|---|---|---|---|---|---|
| 磁束密度 | 400 µT | - | 測定値 | - | - |
The implanted cells differentiated into dopaminergic neurons and sporadically settled in the substantia nigra pars compacta.
Motor asymmetry was significantly increased in Parkinson's disease model rats (group 3) compared to the control group (group 1). However, Parkinson's disease model rats with implantation of stem cells that were exposed to a 40 µT magnetic field for a week (group 4) showed a significantly reduced motor asymmetry after 4 weeks and Parkinson's disease model rats with implantation stem cells that were exposed to a 400 µT magnetic field (group 5) showed significantly reduced motor asymmetry after 2 and 4 weeks compared to group 3.
The BDNF concentration in the brain was significantly increased in group 5 compared to group 3, which in turn showed a lower value than the control group. In the serum, groups 3-6 showed increased BDNF values compared to the control group and no effect of the magnetic field was found.
The number of dopaminergic neurons was significantly decreased in groups 3 compared to the control group (group 2 showed no difference to control). However, group 5 showed a significantly increased number of dopaminergic neurons compared to group 3.
The authors conclude that exposure of rat mesenchymal stem cells to a 50 Hz magnetic field and a subsequent implantation into brains of Parkinson's disease model rats might be beneficial in Parkinson's disease.
このウェブサイトはクッキー（Cookies）を使って、最善のブラウジングエクスペリエンスを提供しています。あなたがこのウェブサイトを継続して使用することで、私たちがクッキーを使用することを許可することになります。
