研究のタイプ: 医学／生物学の研究 (experimental study)

[ラットのアルツハイマー病モデルにおける空間記憶障害と海馬損傷の電磁界ばく露による改善] med./bio.

Improvement of spatial memory disorder and hippocampal damage by exposure to electromagnetic fields in an Alzheimer's disease rat model

著者: Liu X, Zuo H, Wang D, Peng R, Song T, Wang S, Xu X, Gao Y, Li Y, Wang S, Wang L, Zhao L
掲載誌: PLoS One 2015; 10 (5): e0126963
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この研究は、超低周波磁界ばく露（ELF-EMF：50Hz、400µT、60日間）を、D-ガラクトースの腹腔内投与（50mg/kg、1日1回、42日間）およびアミロイドβ25-35の海馬両側への単回注入（片側当たり5μl）と組み合わせ、ELF-EMFばく露がアルツハイマー病（AD）の発症に与える影響をモーリスの水迷路試験、病理学的分析、比較プロテオミクス解析により検討した。その結果、ELF-EMFばく露群では、体重増加が遅れ、ADラットの認知的、臨床病理的症状が部分的に改善された；差別的なプロテオミック解析は前述の影響に関係する結果を示した､と報告している。

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研究目的（著者による）

The effects of exposure of Alzheimer's disease model rats to a 50 Hz magnetic field on cognitive functions, histopathology and protein expression in the brain should be investigated.

詳細情報

Rats were divided into 4 groups: 1) exposure of healthy rats (n=15), 2) exposure of Alzheimer's disease model rats (n=14), 3) non-exposed Alzheimer's disease model rats (n=15), 4) non-exposed healthy rats (n=13; control group).
Rats were examined up to 5 days after termination of exposure.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 24 hours/day for 60 days

General information

background magnetic field in room for non-exposed animals was below 400 nT during exposure

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 24 hours/day for 60 days
ばく露装置
ばく露の発生源／構造
チャンバの詳細 boxes with 20 cm height and 50 cm square bottom
ばく露装置の詳細 two parallel coaxial circular coils with 70 cm height and 140 cm diameter were used and two modular breeding devices each comprising 4 boxes with 8 animals per box were used; MF intensity in breeding device was attenuating ringlike but was unchanged longitudal along the axis of the coils
Additional information non-exposed animals were kept in a separate room on the same floor as the exposed animals; both rooms shared the same ventilation system and humidity, light, temperature and air quality of the both rooms was equal
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 420 µT maximum 測定値 - -
磁束密度 400 µT - 測定値 - at 25 cm distance to point of max value
磁束密度 380 µT - 測定値 - at the edge of the box

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露前
  • ばく露中
  • ばく露後

研究の主なアウトカム（著者による）

All rats exposed to the magnetic field (groups 1 and 2) showed a significantly delayed weight gain compared to non-exposed rats (3 and 4).
In the Morris water maze, a partial improvement of the spatial memory and learning was observed in Alzheimer's disease model rats exposed to the magnetic field (group 2) compared to Alzheimer's disease model rats without exposure (group 3).
Histopathological damages were found in groups 1-3 15 minutes after the end of exposure, but the groups exposed to the magnetic field (groups 1 and 2) showed a partial recovery after 5 further days.
The expression rates of several proteins were significantly different in the groups 1-3 compared to the control group and in exposed Alzheimer's disease model rats (group 2) compared to non-exposed model rats (group 3). Identified proteins are involved in synaptic transmission, oxidative stress, protein degradation, energy metabolism, Tau protein aggregation and inflammation processes.
The authors conclude that exposure to a 50 Hz magnetic field could improve cognitive and histopathological symptoms of Alzheimer's disease model rats and that several molecular clues for further research could be identified.

研究の種別:

研究助成

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