この研究は、被験者(n=124)から採取した精子の運動性、DNA断片化、クラステリン(CLU)遺伝子発現に対する携帯電話からの無線周波電磁界(RF-EMF)ばく露のイン・ビトロでの影響を調べた。まず採取した精子を分析し、正常精子(N群n=26)、精子無力症(A群n=32)、精子奇形無力症(AT群n=31)、乏精子・精子奇形無力症(OAT群n=35)に分け、試各サンプルは2分されて、RF-EMFばく露および無ばく露処置を1時間受けた。ばく露には携帯電話を用いた。処置の前および直後に、精子の運動性、アクロシン活性、DNA断片化、クラステリン遺伝子発現を測定した。その結果、無ばく露群に比べ、ばく露群では精子の運動性、アクロシン活性が低下し、DNA断片化、クラステリン遺伝子発現が増加した;その影響の大きさは、OAT群>AT群>A群>N群であった、と報告している。
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The effects of exposure of human sperm to an 850 MHz electromagnetic field from a mobile phone on different sperm parameters should be investigated.
Clusterin is a protein, which is overexpressed in stressed tissues and appears to be potentially related pathophysiological processes. Acrosin is the major proteinase present in the acrosome of mature spermatozoa, and it is important for fertilization.
Semen samples were collected from 124 donors. Each sample was analyzed in respect of the semen quality according to WHO guidelines. Afterwards, samples were divided into the following groups: 1) normal sperm quality (n=26), 2) decreased sperm motility (n=32), 3) decreased sperm motility and increased rate of sperm malformations (n=31) and 4) severely decreased sperm motility and malformations of sperms (n=35). Each sample was divided into two portions: one for exposure to the electromagnetic field and one without exposure as the control group.
all samples were kept and exposed at room temperature
In group 1 (normal sperm quality), the gene expression and protein expression of clusterin as well as the DNA fragmentation were significantly increased in sperms exposed to the electromagnetic field compared to the control group.
In all other groups (2-4, with pre-experimental sperm quality deficiencies), sperm motility and acrosin enzyme activity were significantly decreased and the gene expression and protein expression of clusterin as well as the DNA fragmentation were significantly increased compared to the control group. A trend towards enhanced effects in sperms with more severe pre-experimental sperm quality deficiencies could be observed.
The authors conclude that exposure of human sperm to an 850 MHz electromagnetic field from a mobile phone could have detrimental effects on sperms.
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