研究のタイプ: 医学／生物学の研究 (experimental study)

[ラットの血液、脾臓機能、血清化学に対する20 MHz無線周波放射の影響] med./bio.

Effects of 20-MHz radiofrequency radiation on rat hematology, splenic function, and serum chemistry

著者: Wong LS, Merritt JH, Kiel JL
掲載誌: Radiat Res 1985; 103 (2): 186-195
RISフォーマットで参考文献をダウンロード

この研究は、Sprague-DawleyラットにTEMチャンバー内で20MHz（HFバンド）連続波ラジオ波（RFR）に6時間/日、5日間/週、6週間ばく露し、その影響を調べた。平均電界強度は2686±164V / m（平均+/- SD）であり、計算された比吸収率は0.3W / kgであった。その結果、ばく露開始から8,22,39および42日目に無作為抽出されて屠殺されたラットの体重、脾臓細胞密度、赤血球および白血球の数、ヘマトクリット、ヘモグロビン、メトヘモグロビン、赤血球脆弱性、ビリルビン、クレアチニン、SGPT、アルカリホスファターゼ、カルシウム、ナトリウム、カリウム、および脾臓細胞の化学発光において、対照群との有意差は示されなかった；脾臓質量の差は、22日目のばく露群と対照群の比較においてのみ有意であった（pは0.05未満）；脾臓対体重比の差は、22日目および39日目のばく露群と対照群の比較においてのみ有意であった（P：0.05未満および0.025未満）などの知見が得られた。ただし、実験に用いたラットの組織学的検査により、呼吸器マイコプラズマ症に特徴的な組織壊死が明らかになったため、追試実験を別のラットセットで実施した結果、脾臓パラメータおよび脾臓細胞の過酸化活性に有意差は認められなかった；これらの動物の組織学的検査では、マイコプラズマ感染の証拠は示されなかった；最初の実験で、ばく露動物と対照動物との間に観察された差異は、RFRへのばく露ではなく呼吸器マイコプラズマ症の結果であると思われる、報告している。

The detailed summary of this article is not available in your language or incomplete. Would you like to see a complete translation of the summary? Then please contact us →

研究目的（著者による）

To investigate the effects of 20 MHz radiofrequency radiation on rat hematology, spleen function, and serum chemistry.

詳細情報

Two different experiments with separate sets of rats but under identical exposure levels, each with unexposed controls were carried out.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 20 MHz
Modulation type: CW
ばく露時間: repeated daily exposure, 6 h/day, 5 days/week, for 8, 22 and 39 days
Experiment 1
  • 電界強度: 2,686.5 V/m mean (± 163.6 V/m)
  • 電力密度: 1,920 mW/cm² mean
  • SAR: 0.26 W/kg mean (whole body) (worst case 0.3 W/kg)
ばく露2: 20 MHz
Modulation type: CW
ばく露時間: repeated daily exposure, 6 h/day, 5 days/week, for 6 weeks
Experiment 2

ばく露1

主たる特性
周波数 20 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 repeated daily exposure, 6 h/day, 5 days/week, for 8, 22 and 39 days
Additional information Experiment 1
Additional information Experiment 1
Modulation
Modulation type CW
ばく露装置
ばく露の発生源／構造
  • TEMセル
  • 4.75 x 9.25 x 30 ft with a 6 ft wide center conductor
チャンバの詳細 Two hundred male rats (280-300 g) were divided into 40 groups and housed, five rats each, in plastic cages of 55 x 31.5 x 20 cm. Twenty cages were randomly selected for control and 20 for exposure. Ambient conditions were similar for control and exposed groups.
ばく露装置の詳細 Cages were spaced 30 cm apart and were rotated through all positions to avoid continuous exposure of a particular cage to a field higher or lower than average. Empty cages were used to fill positions from which cages of sample rats had been removed.
Sham exposure A sham exposure was conducted.
Additional information Control cages were placed in a holding rack immediately outside the TEM chamber during exposure periods.
パラメータ
測定量 値 種別 Method Mass 備考
電界強度 2,686.5 V/m mean 測定値 - ± 163.6 V/m
電力密度 1,920 mW/cm² mean 計算値 - -
SAR 0.26 W/kg mean 計算値 whole body worst case 0.3 W/kg
測定および計算の詳細

Fields were measured at each of the 20 cage sites using a 10-cm dipole and loop antennas. SARs could not be measured because of the low power absorption of the live, moving animals but were calculated for a 320-g rat according to [Durney et al., 1980] to be 0.12 W/kg in H-polarization, 0.17 W/kg in K-polarization and 2.49 W/kg in E-polarization. The maximum SAR was averaged from the percentage of time spent in each orientation (less than 5% upright) and the worst case was determined for all animals huddled together approximated as a single tissue mass.

ばく露2

主たる特性
周波数 20 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 repeated daily exposure, 6 h/day, 5 days/week, for 6 weeks
Additional information Experiment 2
Additional information Experiment 2
Modulation
Modulation type CW
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
チャンバの詳細 Twenty-four male rats were randomly divided into three control and three exposed groups of four animals each. Each rat was housed in a separate cage.
Sham exposure A sham exposure was conducted.
Additional information The treatment of the groups was staggered by 1-week intervals.
パラメータ
測定量 値 種別 Method Mass 備考
電界強度 2,686.5 V/m mean 測定値 - ± 163.6 V/m
電力密度 1,920 mW/cm² mean 計算値 - -
SAR 0.26 W/kg mean 計算値 whole body -

Reference articles

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
研究対象とした臓器系:
  • blood, spleen
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

Overall no significant differences between exposed and control animals were found.
In the first experimental group differences in spleen weight and in the number of erythrocytes and hemoglobin were observed. But these findings could rather be ascribed to an mycoplasmas infection of the animals than to exposure.

研究の種別:

研究助成

関連論文