この研究は、活性酸素種（ROS）の過剰産生を伴う虚血再灌流（I/R）傷害での心筋損傷が、低周波パルス磁界（LFMF）によるROS調節を介して防止できるか否かを調べた。生体でのI/R傷害を模擬するため、新生仔ラットから摘出した心臓の心筋細胞を培養し、低酸素後再酸素（H/R）処置を行った。H/R処置の前または後に、磁界治療器からのLFMFを8条件（磁界強度1.5mT、3.0mT、4.5mT、6.0mT ；周波数15Hz、20Hz）で、1、3、5時間ばく露した。その結果、H/R処置は心筋細胞のアポトーシスを生じ、生存率を低下させたが、H/R処置の前または後のLFMFばく露は有意に､アポトーシスを阻害し､生存率を改善した；LFMFは、H/R処置により生じたROS産生を抑制し、同時にNADPHオキシダーゼ活性も低下させた；LFMFは、NOの産生上昇、NO/ONOO-バランスの上昇を起こしたが、この防護的作用は血管内皮型NO合成酵素のリン酸化を介したものであった､と報告している。
Full text available at PubMed Central : http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3839120
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To investigate whether low frequency magnetic fields can protect cardiac rat myocytes from ischemia re-perfusion injury and to study its underlying molecular mechanisms.
Reconstitution of the blood perfusion in the heart (re-perfusion) can be necessary in the treatment of the acute coronary syndrom (for example myocardial infarction). However, re-perfusion strategies may result in so called "re-perfusion injuries". Those damages are associated with the overproduction of reactive oxygen species. Low frequency pulsed magnetic fields are suggested to prevent the overproduction of reactive oxygen species. Here, the hypoxia/reoxygenation treatment simulates the re-perfusion.
Several groups were examined: 1.) control group, 2.) hypoxia/reoxygenation treatment, 3.) 8 different magnetic field exposure groups. Cell cultures were exposed to the magnetic field for 1, 3 or 5 hours before or after the hypoxia/reoxygenation treatment.
For the examination of the generation reactive oxygen species, positive controls were performed. For the generation of nitric oxide, the enzyme activity of the endothelial nitric oxide synthase was inhibited with NG-nitroarginine methyl ester. For the generation of superoxide, hydrogen peroxide was used as a positive control.
| ばく露 | パラメータ |
|---|---|
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ばく露1:
15 Hz
ばく露時間:
1, 3 or 5 hours
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ばく露2:
20 Hz
ばく露時間:
1, 3 or 5 hours
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ばく露3:
15 Hz
ばく露時間:
1, 3 or 5 hours
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ばく露4:
20 Hz
ばく露時間:
1, 3 or 5 hours
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ばく露5:
15 Hz
ばく露時間:
1, 3 or 5 hours
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ばく露6:
20 Hz
ばく露時間:
1, 3 or 5 hours
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ばく露7:
15 Hz
ばく露時間:
1, 3 or 5 hours
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ばく露8:
20 Hz
ばく露時間:
1, 3 or 5 hours
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Hypoxia/reoxygenation treatment alone (group 2) significantly increased apoptosis und decreased cell viability compared to control cell cultures. However, the magnetic field exposure, especially at 4.5 mT/15 Hz and 3 hours before or after the hypoxia/reoxygenation treatment significantly decreased the apoptosis and enhanced cell viability compared to cell cultures with hypoxia/reoxygenation treatment alone. The levels of superoxide und peroxynitrite and the enzyme activity of NADPH oxidase were significantly decreased in the magnetic field exposed groups compared to group 2 (independent whether exposure was before or after hypoxia/reoxygenation treatment) while the level of nitric oxide and the protein expression of the phosphorylated endothelial nitric oxide synthase were significantly increased.
The authors conclude, that low frequency magnetic fields could protect cardiac rat myocytes against ischemia re-perfusion injury via a modulation of the reactive oxygen species formation. Hence, low frequency magnetic fields might present a promising tool for the prevention of cardiac ischemia re-perfusion injuries.
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