研究のタイプ: 医学/生物学の研究 (experimental study)

[培養ヒト細胞における1800MHz GSM無線周波ばく露および化学的突然変異原の共遺伝毒性の評価] med./bio.

Evaluation of the co-genotoxic effects of 1800 MHz GSM radiofrequency exposure and a chemical mutagen in cultured human cells

掲載誌: CR physique 2010; 11 (9-10): 613-621

この研究は、既知の化学変異原(4-ニトロキノリン-N-オキシド:4NQO)と1800 MHz無線周波GSM信号の組み合わせばく露が、ヒトTHP1細胞に及ぼす影響を調べた。コメットおよびγ-H2AXアッセイを使用して、DNA損傷を評価した。その結果、ばく露中(2時間)、細胞培養物の加熱は認められなかった;培地のSAR値が2 - 16 W / kgの範囲の電磁界ばく露は、4NQOによって誘発されるDNA損傷を増加させなかった;一方、少なくとも4°Cの温度上昇があると、DNA鎖切断の数は増加した;以上の知見から、加熱を引き起こさない程度のばく露レベルの無線周波電磁界に共遺伝毒性効果は見られないことが示唆された、と報告している。

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研究目的(著者による)

To study the effect of a 1800 MHz radiofrequency GSM signal combined with a known chemical mutagen (4-nitroquinoline-N-oxide: 4NQO) on human cells.

詳細情報

No heating of the cell cultures was detected during exposure. Additional experiments were performed at 37, 39, 41 or 43°C (2 h, with 4NQO).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1,800 MHz
Modulation type: pulsed
ばく露時間: continuous for 2 h

General information

cells were treated in four groups: i) sham exposure ii) EMF exposure iii) 1.8 µM 4NQO (4-nitroquinoline-N-oxide) + sham exposure iv) 1.8 µM 4NQO + EMF exposure

ばく露1

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
特性
  • far field
ばく露時間 continuous for 2 h
Modulation
Modulation type pulsed
Duty cycle 12.5 %
Repetition frequency 217 Hz
ばく露装置
ばく露の発生源/構造
Distance between exposed object and exposure source 1 m
チャンバの詳細 three identical exposure chambers used - two for simultaneous exposures and one for sham exposure
ばく露装置の詳細 cells placed in Petri dishes inside thermoregulated Plexiglas incubators which were slowly rotated and positioned each inside an anechoic chamber; temperature inside the incubator 37°C; layer of small glass balls with a diameter of 1.5 mm placed inside the Petri dishes for efficient heat conduction; rectangular horn antenna positioned above the incubator;
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 2 W/kg - - - -
SAR 4 W/kg - - - -
SAR 8 W/kg - - - -
SAR 16 W/kg - - - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The exposure of cells to electromagnetic fields with SARs of 2 to 16 W/kg did not increase the DNA damage induced by 4NQO, whereas the number of DNA strand breaks increased with a temperature rise of at least 4 °C. In conclusion, no co-genotoxic effect of radiofrequency electromagnetic field exposure was found at levels of exposure that did not induce heating.

研究の種別:

研究助成

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