研究のタイプ: 医学／生物学の研究 (experimental study)

[急性無線周波放射は細胞周期、細胞遊走、浸潤性に影響を与えない] med./bio.

Acute radio frequency irradiation does not affect cell cycle, cellular migration, and invasion

著者: Lee JJ, Kwak HJ, Lee YM, Lee JW, Park MJ, Ko YG, Choi HD, Kim N, Pack JK, Hong SI, Lee JS
掲載誌: Bioelectromagnetics 2008; 29 (8): 615-625
RISフォーマットで参考文献をダウンロード

目的：RF放射へのばく露が、細胞周期分布、細胞の浸潤性、細胞遊走性に検出可能な影響を与えるか否かを明らかにすること。方法：マウスの線維芽細胞NIH3T3に、849 MHzのRF放射を、平均SAR値2または10W/kg のどちらかで、1時間ばく露を1回行うか、1時間ばく露を１日1回、3日連続で行った。細胞を入れたペトリ皿を置いたばく露空洞内は水を循環させ、ばく露中に温度変化がないように保たれた。細胞周期分布は、ばく露終了後24時間および48時間経過した時点で、フローサイトメトリ－によって解析された。細胞の浸潤性と遊走性は、それぞれMatrigel浸潤アッセイ、Transwell遊走アッセイによって評価された。結果：細胞周期分布、浸潤性、遊走性について、ばく露群と擬似ばく露群との間に統計学的に有意な差異は検出されなかった。ポジティブコントロールに用いた放射線ばく露群では、細胞周期分布、浸潤性、遊走性の特性に著しい変化が現れた。結論：平均SAR値2または10W/kgの849 MHzのRF放射は細胞周期分布、細胞の浸潤性、遊走性に検出可能な影響を与えないことが示された。

The detailed summary of this article is not available in your language or incomplete. Would you like to see a complete translation of the summary? Then please contact us →

研究目的（著者による）

To study whether radiofrequency exposure exerts detectable effects on cell cycle distribution, cellular invasion, and migration.

詳細情報

Radiofrequency exposure was conducted under isothermal conditions. For the positive control group experiments, the cells were exposed to various doses of gamma radiation.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 849 MHz
ばく露時間: continuous for 1 h
  • SAR: 2 W/kg spatial average
  • SAR: 10 W/kg spatial average
ばく露2: 849 MHz
ばく露時間: repeated daily exposure, 1 h/day, for 3 days
  • SAR: 2 W/kg spatial average
  • SAR: 10 W/kg spatial average

ばく露1

主たる特性
周波数 849 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 1 h
Modulation
Modulation type cf. additional information
Additional information

real CDMA test signal
ばく露装置
ばく露の発生源／構造
チャンバの詳細 The exposure system was fed with a λ/8 monopole antenna (33 mm long) at the λ/4 position of the top plate of the cavity to excite only the fundamental TE102 mode, and it allowed for the uniform exposure of one 100-mm culture plate. The gas from an incubator was circulated throughout the chamber to maintain CO2 density and humidity. Additionally, cooling water was circulated through the bottom of the cavity to maintain the temperature at 37 ± 0.3°C.
ばく露装置の詳細 The cells were exposed in a 100-mm Petri dish (I.D. 85 mm) in 18 ml of culture medium at 4 mm height.
Sham exposure A sham exposure was conducted.
Additional information The exposure system was warmed up for 30 min to equilibrate it prior to RF exposure.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 2 W/kg spatial average 測定値 - -
SAR 10 W/kg spatial average 測定値 - -
Additional parameter details

For positive control, the cells were exposed to various doses (0.2-10 Gy) of 137Cs gamma radiation at a dose rate of 3.81 Gy/min.

測定および計算の詳細

SAR inside the exposed medium was characterized by numerical simulations using the FDTD method and also by measurements using thermal probes located at nine points inside a Petri dish. When comparing the measurement data with the simulation results, the inevitable modelling errors seem to be rather big, but they show a similar trend for each position. The reported SARs correspond to the mean of the values measured (?) at three points along a radial path from the center to the edge of the dish.

ばく露2

主たる特性
周波数 849 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 repeated daily exposure, 1 h/day, for 3 days
Modulation
Modulation type cf. additional information
Additional information

real CDMA test signal
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 2 W/kg spatial average 測定値 - -
SAR 10 W/kg spatial average 測定値 - -

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

The data showed that 849 MHz radiofrequency exposure exerts no detectable effects on cell cycle distribution, cellular migration, or invasion at average SAR values of 2 or 10 W/kg.

研究の種別:

研究助成

関連論文