この研究は、発達中の神経系の実験試料としてラットの初期皮質細胞培養を用い、イン・ビトロでの50Hzの超低周波電磁界（ELF-EMF：矩形波パルス、0-1000μT、7日間ばく露）への発生期ばく露が細胞生存、カルシウム恒常性、神経突起伸長、自発的な神経細胞活動に及ぼす影響を調べた。その結果、細胞生存への影響は無かった；脱分極あるいはグルタミン酸誘発性の細胞内カルシウム濃度（[Ca2+]i）上昇は1μTばく露において若干大きくなったが、基礎的[Ca2+]iおよび刺激誘発[Ca2+]iは1000μTばく露において僅かに抑制された；神経突起の長さは、100μTまでは影響がなく、1000μTで増加した：全体としては、発生神経毒性はほぼ見られず、1000μTで限定的な影響が見られた、と報告している。
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The neurotoxic effects of exposure to a 50 Hz magnetic field on the development of primary rat cortical neurons should be investigated.
Cells were divided into the following groups: exposure to a magnetic field of 1) 1 µT, 2) 10 µT, 3) 100 µT, 4) 1000 µT and 5) sham exposure.
All data were derived from at least 3 independent experiments.
| 周波数 | 50 Hz |
|---|---|
| タイプ |
|
| 波形 |
|
| ばく露時間 | continuous for 7 days |
| Additional information | block-pulsed magnetic field with a main frequency of 50 Hz (<10% harmonics) |
| ばく露の発生源／構造 | |
|---|---|
| チャンバの詳細 | incubator |
| ばく露装置の詳細 | double copper wired solenoid coils fitted into an incubator; coils consisted of one continuous copper conductor; the system generated AC and DC vertical field components |
| Sham exposure | A sham exposure was conducted. |
| Additional information | sham exposed cells were placed in a second incubator fitted with the same double copper wired solenoid coils, but switched off |
| 測定量 | 値 | 種別 | Method | Mass | 備考 |
|---|---|---|---|---|---|
| 磁束密度 | 1 µT | effective value | 測定値 | - | - |
The basal intracellular calcium concentration (unstimulated) was slightly but significantly decreased in group 4 (1000 µT) compared to the sham exposure group. The stimulated intracellular calcium concentration was significantly increased in group 1 (1 µT) and significantly decreased in group 3 (100 µT) and group 4 compared to the sham exposure group.
The axon outgrowth was significantly increased in group 4 compared to the sham exposure group.
Exposure to the magnetic field had no effect on cell viability and the electrical activity of the cells.
The authors conclude that exposure to a 50 Hz magnetic field has only limited neurotoxic effects on the development of primary rat cortical neurons in the case of strong fields.
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