この研究は、900MHz電磁界（EMF）に1日1、2、4時間、30日連続でばく露した成獣SD雄ラットから全摘した精巣の標本のプロテオームを解析した。その結果、擬似ばく露群、またはばく露群でのみ見られる合計13個のタンパク質をMALDI-TOF/TOF-MSで同定した；これらには、熱ショックタンパク質、スーパーオキシドジスムターゼ、ペルオキシレドキシン-1などが合った、と報告している。
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The effects of a whole body exposure of rats to a 900 MHz electromagnetic field on the protein expression pattern in the testes should be investigated.
20 rats were divided into the following groups (n=5 each group): 1) sham exposure, 2) 1 h exposure, 3) 2 h exposure and 4) 4 h exposure. Experiments were conducted at least in triplicates.
| ばく露 | パラメータ |
|---|---|
|
ばく露1:
900 MHz
ばく露時間:
repeated daily exposure for 1 h on 30 consecutive days
|
|
|
ばく露2:
900 MHz
ばく露時間:
repeated daily exposure for 2 h on 30 consecutive days
|
|
|
ばく露3:
900 MHz
ばく露時間:
repeated daily exposure for 4 h on 30 consecutive days
|
| 周波数 | 900 MHz |
|---|---|
| タイプ |
|
| ばく露時間 | repeated daily exposure for 1 h on 30 consecutive days |
| チャンバの詳細 | 5 freely moving rats were exposed in a pure transparent polycarbonate cage of 42 cm Œ 26.5 cm Œ 15 cm near the antenna |
|---|---|
| ばく露装置の詳細 | temperature was 22 ± 2°C and relative humidity was 50% |
| Sham exposure | A sham exposure was conducted. |
| 周波数 | 900 MHz |
|---|---|
| タイプ |
|
| ばく露時間 | repeated daily exposure for 2 h on 30 consecutive days |
| ばく露の発生源／構造 |
|
|---|---|
| Sham exposure | A sham exposure was conducted. |
| 周波数 | 900 MHz |
|---|---|
| タイプ |
|
| ばく露時間 | repeated daily exposure for 4 h on 30 consecutive days |
| ばく露の発生源／構造 |
|
|---|---|
| Sham exposure | A sham exposure was conducted. |
No significant differences were found in weight and total protein concentration of testes between the groups. Two-dimensional gel electrophoresis revealed 16 protein spots that were found only in the sham-exposed group, while 18 protein spots existed only in the exposed groups. No clear differences in the protein expression pattern were found between the exposure groups. 6 proteins which were found only in the sham-exposed group and 7 proteins which were found only in the exposed groups were identified, e.g. several heat shock proteins in the exposed groups and a superoxide dismutase in the sham exposure.
The authors conclude that an exposure of rats to a 900 MHz electromagnetic field could induce the expression or the absence of proteins related to stress and stress protection, respectively, in the testes. Further studies are needed to confirm and extend the results of this preliminary study.
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