この研究は、緑色蛍光タンパクの溶液の蛍光に対するマイクロ波放射および通常の熱の影響を比較した。マイクロ波のばく露には、入力電力250mWで動作する8.5GHzのマイクロ波アプリケータからの近傍界を用いた。マイクロ波ばく露または通常加熱の場合について、溶液温、緑色蛍光タンパクからの蛍光の強度とスペクトラムを測定した。その結果、どちらの場合も、蛍光の強度低下、スペクトラムの赤色方向へのシフトが見られた、と報告している。
The detailed summary of this article is not available in your language or incomplete. Would you like to see a complete translation of the summary? Then please contact us →
To compare the effect of microwave irradiation and of conventional heating on the fluorescence of green fluorescent protein (in solution).
An aqueous egg-white solution and a liquid crystal indicator were also used to test temperature measurement.
| ばく露 | パラメータ |
|---|---|
|
ばく露1:
8.53 GHz
Modulation type:
CW
ばく露時間:
intermittent, about 200 s on/off, at each of 6 decreasing power levels, for a total of 3200 s
|
|
|
ばく露2:
8.53 GHz
Modulation type:
pulsed
ばく露時間:
intermittent, about 200 s on/off, at CW and each of 3 decreasing duty cycles, for a total of 1600 s
|
|
|
ばく露3:
250 MHz–20 GHz
Modulation type:
CW
ばく露時間:
continuous, with increasing frequencies, 10 s at each step, for a total of 4000 s
|
|
The EGFP fluorescence was excited by a 488-nm Argon laser with its beam directed across a transparent 2-mm diameter glass pipette (with 150-µm walls) containing the sample. The temperature dependence of the EGFP fluorescence was measured by a thermocouple in close vicinity of the laser beam. In the absence of MW radiation, the sample was heated between 22 and 43°C using a water bath or a thermal resistor.
| 周波数 | 8.53 GHz |
|---|---|
| タイプ |
|
| 特性 |
|
| ばく露時間 | intermittent, about 200 s on/off, at each of 6 decreasing power levels, for a total of 3200 s |
| Modulation type | CW |
|---|
| ばく露の発生源／構造 |
|
|---|---|
| Distance between exposed object and exposure source | 0.25 mm |
| チャンバの詳細 | The MW applicator was brought to a distance of 100 µm above the glass pipette in such a way that the probe's apex aimed directly at the laser illuminated region. |
| ばく露装置の詳細 | For narrowband measurements, a special 8.5 GHz applicator was used, based on a narrow rectangular aperture microfabricated on the convex surface of the sapphire dielectric resonator. The operating frequency of the probe was 8.53 GHz, and the bandwidth with the sample present was typically 0.3 GHz. |
| Additional information | The incident MW power was switched on and off, decreasing from 250 mW to 20 mW, and finally returning to 250 mW. In a different experiment, the MW power was varied in steps of 0.1 dBm with a dwell time of 2 s. |
| 周波数 | 8.53 GHz |
|---|---|
| タイプ |
|
| 特性 |
|
| ばく露時間 | intermittent, about 200 s on/off, at CW and each of 3 decreasing duty cycles, for a total of 1600 s |
| Modulation type | pulsed |
|---|---|
| Pulse width | 1 µs |
| Additional information |
pulse periods of 2, 5 and 10 µs, resulting in duty cycles of 50, 20, and 10% |
| ばく露の発生源／構造 |
|
|---|---|
| Additional information | The incident MW power was switched on and off, with the duty cycle decreasing from CW to 10%. |
| 測定量 | 値 | 種別 | Method | Mass | 備考 |
|---|---|---|---|---|---|
| 電力 | 250 mW | - | - | - | - |
| 周波数 | 250 MHz–20 GHz |
|---|---|
| タイプ |
|
| 特性 |
|
| ばく露時間 | continuous, with increasing frequencies, 10 s at each step, for a total of 4000 s |
| Modulation type | CW |
|---|
| 測定量 | 値 | 種別 | Method | Mass | 備考 |
|---|---|---|---|---|---|
| 電力 | 250 mW | - | - | - | - |
A specific microwave effect on the fluorescence of the green fluorescent protein molecule which is distinguishable from conventional heating is found. In both cases the fluorescence intensity decreased, but the effect of microwave exposure on the fluorescence is stronger than expected from thermal physics considerations. Although the microwave irradiation heated the solution, the microwave-induced changes in fluorescence cannot be explained by heating alone.
このウェブサイトはクッキー（Cookies）を使って、最善のブラウジングエクスペリエンスを提供しています。あなたがこのウェブサイトを継続して使用することで、私たちがクッキーを使用することを許可することになります。
