この研究は、パルス化RF電磁界（2.45 GHz）が、培養されたヒトHL-60細胞に遺伝子発現の変化を引き起こすか否かを調べた。遺伝子発現のシリアル解析（SAGE）法を用いて、ゲノムレベルでの遺伝子発現に対するRF効果を測定した。結果として、22の遺伝子が2時間のばく露後に発現を変化させることが観察された；影響を受ける遺伝子の数は、6時間のばく露後に759に増加した；発現増加した遺伝子の中には、アポトーシス関連遺伝子が含まれ、発現低下した遺伝子の中には、細胞周期遺伝子があった；熱ショック遺伝子の発現に有意な増加は観察されなかった、と報告している。
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To study whether radiofrequency fields can cause changes in gene expression in cultured human cells. The authors aimed to provide genome-wide coverage of the expressed genes regardless their functional categories in the radiofrequency treated cells to address if radiofrequency has biological effects.
The RF exposure system has been described in detail [Gerber et al., manuscript in preparation].
| 周波数 | 2.45 GHz |
|---|---|
| タイプ |
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| 特性 |
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| ばく露時間 | continuous for 2 or 6 h |
| Modulation type | pulsed |
|---|---|
| Pulse width | 155 µs |
| Duty cycle | 7.5 % |
| ばく露の発生源／構造 |
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|---|---|
| チャンバの詳細 | The waveguide was filled with a 5% air-CO2 mixture through a hole in its top surface. The bottom of the waveguide was maintained at 37 °C by temperature-controlled water circulating in direct contact with the brass surface through an exterior plastic channel glued to it. |
| ばく露装置の詳細 | A 25-ml culture flask containing 10 ml of cell suspension was placed inside the waveguide. The bottom of the flask was ground flat and coated with mineral oil to obtain good thermal conduction. The temperature in the bottom surface of the flask monitored by two Luxtron probes was maintained at 37.2 ± 0.2 °C. |
| Sham exposure | A sham exposure was conducted. |
| Additional information | The cells were allowed to settle down to the bottom of the flask to form a monolayer before exposure. Sham exposure was performed in an identical waveguide for 2 h only. |
The authors revealed that 221 genes altered their expression after a 2 h exposure. The number of affected genes increased to 759 after a 6 h exposure. Functional classification of the affected genes showed that apoptosis-related genes were among the upregulated ones and the cell cycle genes among the downregulated ones. No significant increase in the expression of heat shock protein genes was found.
These data suggest that the radiofrequency fields at 2.45 GHz can alter gene expression in cultured human cells through non-thermal mechanism.
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