【目的】ヒト好中球のcAMP濃度勾配にしたがって移動する能力（走化性）に対するRF電磁界の影響の研究。【方法】健常ドナーからの血液を、様々な温度および約0.4V/mの900MHz電磁界にばく露した。【結果】好中球の速度は、RFばく露なしの場合、35度から40度までは上昇し、40度でピークとなり、40度以上では低下することを観察した。900MHz電磁界ばく露を行った場合、同じ温度で観察された速度以上に上昇し、速度の最大値は種々の温度で観察された最大値をおよそ50％上回った。RFばく露がもたらす温度変化の計算値は１マイクロ度以下であった。動きの方向は、濃度勾配および電界方向に沿ったものから、濃度勾配に直角な方向および電界方向へと変化した。RF電磁界への好中球の平均応答時間は約2.5分であった。
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To study the effects of radiofrequency fields on the ability of human neutrophils to follow concentration gradients of cyclic AMP (chemotaxis).
Cell's ability to move depends on the type of leukocyte and its age, the amount of anti-coagulant used, and other unknown variables in the condition of the donors. Blood was drawn of 8 students. The effect of different temperatures (22°C - 43°C) was also studied.
| 周波数 | 900 MHz |
|---|---|
| タイプ |
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| 特性 |
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| ばく露時間 | continuous for about 15 min |
| ばく露の発生源／構造 |
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|---|---|
| チャンバの詳細 | A drop of fluid containing neutrophils was covered with a cover slip to create a layer about 15 µm thick. The cover slip was sealed with Vaseline around the edges to reduce the effects of pressure, temperature, and vibration on the movement of the leukocyte cells and to prevent the sample from drying out. |
| ばく露装置の詳細 | The slide was placed for about 15 min on the microscope stage heated to the desired temperature, thus allowing it to reach steady state. |
| Additional information | The temperature was increased in a series of experiments by one degree from 22 °C to 43 °C both with and without RF exposure. The motion of the exposed cells was observed for about 15 min before and for about 15 min after applying the RF radiation. |
The speed of the neutrophils increased with increasing temperatures from 35°C to 40°C where it peaked and then decreased above 40°C without radiofrequency exposure.
Under 900 MHz radiofrequency field exposure, the speed increased above the value observed at the same temperature, and the maximum speed exceeded the measured value at any temperature by approximately 50%. The calculated temperature change resulting from the radiofrequency exposure was less than one microdegree.
Upon radiofrequency exposure the neutrophils moved perpendicular to the direction along the cAMP gradient and to the applied electrical fields. Without radiofrequency these cells moved toward the cAMP stripe.
The authors conclude that the effects of radiofrequency exposure on neutrophil chemotaxis should be considered for further exploration in larger and more controlled studies.
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