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研究目的（著者による）
The effects of exposure of mouse spermatocyte-derived cells to a 50 Hz magnetic field on the microRNA expression and the possible use of microRNAs as biomarkers for magnetic field exposure should be investigated.
詳細情報
MicroRNAs are small non-coding RNAs, which regulate gene expression at the post-transcriptional level. Mouse spermatocyte-derived cells were investigated to explore the mechanisms of action of possible adverse effects of magnetic fields on the male reproductive system. Cells were divided into the following groups: exposure to a magnetic field of 1) 1 mT, 2) 2 mT and 3) 3 mT. For each group, a separate sham exposure was conducted simultaneously. Except for microarray experiments, all tests were repeated three times.
two rectangular waveguides and two four-coil systems (two coils with 56 windings, two coils with 50 windings) generated a vertical magnetic field inside the chamber; system was composed of two identical exposure chambers (one for exposure, the other for simultaneous sham exposure); environmental conditions were constant (37°C, 5% CO2); exposure started after overnight starvation
Sham exposure
A sham exposure was conducted.
Additional information
the temperature difference between sham exposure and exposure chamber never exceeded 0.3°C
No significant differences were found in cell morphology, cell viability, apoptosis and cell cycle distribution between the exposure and respective sham exposure groups. In the microarrays, 19 microRNAs with a significantly differential expression rate were found in group 1 (1 mT) (7 microRNAs were upregulated, 12 were downregulated) and 36 microRNAs were significantly differentially expressed (9 miRNAs were upregulated, and 27 were downregulated) in group 3 (3 mT) compared to the sham exposure groups. This results were confirmed by the real-time RT-PCR. The pathway analyses showed that the identified microRNAs may regulate circadian rhythms, cytokine-cytokine receptor interactions and the p53 signaling pathway. The authors conclude that exposure of mouse spermatocyte-derived cells to a 50 Hz magnetic field could alter the microRNA expression and that microRNAs could serve as potential biomarkers for magnetic field exposure.
