研究のタイプ: 医学／生物学の研究 (experimental study)

[1800MHz無線周波電磁界へのアストロサイトとミクログリアとで異なる炎症誘発性反応はSTAT3活性化が関与する] med./bio.

Differential Pro-Inflammatory Responses of Astrocytes and Microglia Involve STAT3 Activation in Response to 1800 MHz Radiofrequency Fields

著者: Lu Y, He M, Zhang Y, Xu S, Zhang L, He Y, Chen C, Liu C, Pi H, Yu Z, Zhou Z
掲載誌: PLoS One 2014; 9 (9): e108318
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この研究は、マウスのアストロサイトC8-D1AとミクログリアN9に、転写因子STAT3の阻害剤添加の有無の両条件下で1800MHz無線周波電磁界（RF）のばく露（SARは2 W/kg、ばく露時間は1、3、6、12、24時間）を与え、炎症誘発性反応を調べた。その結果、RFばく露により、アストロサイトとミクログリアとでは、インターロイキン（IL-1β、IL-6）、腫瘍壊死因子（TNF-α）、プロシタグランジン（PGE2）などの発現レベルが異なる炎症誘発性反応が見られた；RFばく露により、ミクログリアではSTAT3が活性化したが、アストロサイトでは活性化されなかった；ミクログリアでは、STAT3阻害剤添加によりRF誘導性の炎症誘発性サイトカインの放出が増加した、と報告している。

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研究目的（著者による）

To investigate the radiofrequency field-induced proinflammatory responses of microglia and astrocytes and the involved molecular mechanisms.

詳細情報

STAT3 (signal transducer and activator of transcription 3) mediates signal transduction from the extracellular environment to the nucleus. To test the potential involvement of STAT3 in the response to radiofrequency fields, the STAT3 inhibitor "Stattic" was partially added to the culture medium 24 hours before the radiofrequency field exposure.
Positive controls were performed via treatment with lipopolysaccharides (1 µg/ml).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1,800 MHz
Modulation type: pulsed
ばく露時間: intermittent for 1, 3, 6, 12, or 24 hours (5 min on and 10 min off)
  • SAR: 2 W/kg mean

ばく露1

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
波形
  • pulsed
ばく露時間 intermittent for 1, 3, 6, 12, or 24 hours (5 min on and 10 min off)
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
Pulse type rectangular
ばく露装置
ばく露の発生源／構造
チャンバの詳細 cells were cultured in 35 mm plastic Petri dishes
ばく露装置の詳細 system consisted of two resonating waveguides; in each waveguide chamber, a Petri dish holder for six dishes ensured that each dish was positioned accurately in the H-field maximum of the standing wave; two ventilation holes were located on the waveguide, a ventilator fan was fixed to one hole to cool the culture medium; waveguides were placed in an incubator to ensure constant environmental conditions (37 ± 0.2°C, 5% CO2, 95% air atmosphere)
Sham exposure A sham exposure was conducted.
Additional information temperature rise due to radiofrequency field was less than 0.05°C
パラメータ
測定量 値 種別 Method Mass 備考
SAR 2 W/kg mean 測定値および計算値 - -

Reference articles

  • Schuderer J et al. (2004): [1800 MHzのインビトロ実験に用いる強力なばく露および環境制御機能を備えた高尖頭SARばく露装置]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム（著者による）

Radiofrequency exposure induced activation of both cell types: After 12 and 24 hours exposure, the protein expression of CD11b in microglia was significantly increased compared to the corresponding sham exposure. In astrocytes, the protein expression of glial fibrillary acidic protein was significantly increased after 24 hours exposure compared to the sham exposure as shown via immunohistochemistry and Western blot.
Microglia and astrocytes exposed to radiofrequency fields showed both proinflammatory responses compared to the sham exposure: However, the expression and release profiles of interleukin-1alpha, tumor necrosis factor-alpha, interleukin-6, prostaglandin E2, nitric oxide, inducible nitric oxide synthase, and cyclooxygenase-2 differed between the two cell types. The expression of STAT 3 was only activated in microglia. Hence, an addition of the STAT3 inhibitor "Stattic" attenuated the exposure induced effects in microglia but not in astrocytes.
The authors conclude that exposure to radiofrequency fields induces differential proinflammatory responses in microglia and astrocytes which could be mediated through different activation of STAT3 in each cell type.

研究の種別:

研究助成

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