研究のタイプ: 医学／生物学の研究 (experimental study)

[1.8 GHz無線周波電磁界にばく露したヒト水晶体上皮細胞の酸化ストレスの研究] med./bio.

Study of Oxidative Stress in Human Lens Epithelial Cells Exposed to 1.8 GHz Radiofrequency Fields

著者: Ni S, Yu Y, Zhang Y, Wu W, Lai K, Yao K
掲載誌: PLoS One 2013; 8 (8): e72370
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この研究は、低レベルの1.8GHz無線周波電磁界（RF）にばく露したヒト水晶体上皮細胞（HLE）のB3細胞株に生じる酸化ストレスを測定し、活性酸素種（ROS）が増加する理由を探索した。HLE B3細胞株をRFばく露群と擬似ばく露群に分け、RFばく露には導波管チャンバー（IT’IS製）を用い、SARの大きさは2、3、4 W/kgとした。ばく露時間0.5、1、1.5時間においてROCレベルを、同6、12、24時間において脂質過酸化の大きさ（MDAレベル）および細胞の生存率を測定した。また、ばく露1時間において、SOD1、SOD2、CAT、GPx1遺伝子のmRNA発現およびSOD1、SOD2、CAT、GPx1タンパクの発現を測定した。その結果、RFばく露群では、擬似ばく露群に比べ、ROSおよびMDAレベルが有意に増加し、細胞生存率、４つの遺伝子のmRNA発現およびタンパク質発現が有意に低下した、と報告している。

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研究目的（著者による）

To investigate possible reasons for the increase of reactive oxygen species formation in human eye lens epithelial cells exposed to low intensity 1.8 GHz radiofrequency fields.

詳細情報

Human eye lens epithelial cells were used in this study because the eye is an important organ frequently exposed to radiofrequency fields from mobile phones.
The cells were sham exposed or exposed to radiofrequency fields with specific absorption rates of 2, 3 or 4 W/kg. Different exposure durations were used: For the detection of reactive oxygen species, cells were exposed for 0.5, 1 or 1.5 hours and for the analysis of the cell viability and lipid peroxidation 6, 12 or 24 hours. The exposure duration for gene expression and protein expression analysis was 1 hour.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1,800 MHz
Modulation type: pulsed
ばく露時間: intermittent (5 minutes field "on"/10 minutes field "off") for up to 24 hours
  • SAR: 2 W/kg mean
ばく露2: 1,800 MHz
Modulation type: pulsed
ばく露時間: intermittent (5 minutes field "on"/10 minutes field "off") for up to 24 hours
  • SAR: 3 W/kg mean
ばく露3: 1,800 MHz
Modulation type: pulsed
ばく露時間: intermittent (5 minutes field "on"/10 minutes field "off") for up to 24 hours
  • SAR: 4 W/kg mean

ばく露1

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
ばく露時間 intermittent (5 minutes field "on"/10 minutes field "off") for up to 24 hours
Modulation
Modulation type pulsed
Duty cycle 12.5 %
Repetition frequency 217 Hz
Pulse type rectangular
Additional information

GSM-like signal
ばく露装置
ばく露の発生源／構造
チャンバの詳細 exposure system consists of exposure-waveguide chamber, sham exposure chamber, signal tower, amplifiers, PC with software and a CO2 incubator at 37°C; temperature difference never exceeded 0.1°C between exposed cells and sham exposed cells
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 2 W/kg mean - - -

ばく露2

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
ばく露時間 intermittent (5 minutes field "on"/10 minutes field "off") for up to 24 hours
Modulation
Modulation type pulsed
Duty cycle 12.5 %
Repetition frequency 217 Hz
Additional information

GSM-like signal
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 3 W/kg mean - - -

ばく露3

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
ばく露時間 intermittent (5 minutes field "on"/10 minutes field "off") for up to 24 hours
Modulation
Modulation type pulsed
Duty cycle 12.5 %
Repetition frequency 217 Hz
Additional information

GSM-like signal
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 4 W/kg mean - - -

Reference articles

  • Xu S et al. (2013): [1800 MHz無線周波電磁界により細胞種依存性のDNA損傷が誘導されるが重大な細胞機能障害にはならない]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

In all exposed cell cultures, the level of reactive oxygen species and the lipid peroxidation were significantly increased compared to the corresponding sham exposed cell cultures, while the cell viability, the gene expression and the protein expression were significantly decreased.
The authors conclude that the observed increase in the oxidative stress level in human eye lens epithelial cells exposed to radiofrequency fields may be due to a down-regulation of antioxidant enzymes.

研究の種別:

研究助成

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