研究のタイプ: 医学／生物学の研究 (experimental study)

[携帯電話からのマイクロ波はヒト幹細胞において分化した細胞よりも強く53BP1フォーカス形成を阻害する：がんリスクとのメカニズム的関連の可能性] med./bio.

Microwaves from Mobile Phones Inhibit 53BP1 Focus Formation in Human Stem Cells Stronger than in Differentiated Cells: Possible Mechanistic Link to Cancer Risk

著者: Belyaev I, Markova E, Malmgren L
掲載誌: Environ Health Perspect 2010; 118 (3): 394-399
RISフォーマットで参考文献をダウンロード

この実験研究は、GSMおよびUMTSの携帯電話からのマイクロ波（MW）が幹細胞中のDNA二重鎖切断（DSB）またはDSBの修復に与える影響を、DSBが発生した位置に形成される53BP1 foci（DNA修復foci）の検出により調べた。ヒト初代線維芽細胞と間葉系幹細胞（MSC）に対し、ばく露（GSM905MHz、同915MHz、UMTS1947.4MHz）および擬似ばく露を1、2、3時間与えた。その結果、GSM915MHzとUMTS1947.4MHzはどちらの細胞にも影響した一方、GSM905MHzは分化細胞であるヒト初代線維芽細胞の53BP1 foci形成を阻害せず、幹細胞にはいくらか影響したと報告している。

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研究目的（著者による）

To study whether microwaves from GSM or UMTS mobile telephones induce double-strand breaks or affect double-strand break DNA repair in stem cells or fibroblasts.

詳細情報

Heat treatment (41°C) was used as positive control for stress response. Gamma radiation (3 Gy) was used as positive control for genotoxic effects.
The measurement parameter of 53BP1 foci inhibition was used to study double-strand breaks. The authors suggest that inhibition of 53BP1 foci formation might be caused by a decrease in accessibility of double-strand breaks to proteins because of stress-induced chromatin condensation or by inhibition of phosphorylation of the 53BP1 protein that is necessary to activate the protein for DNA repair.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 915 MHz
ばく露時間: continuous for 1 h, 2 h or 3 h
GSM 1
ばく露2: 905 MHz
ばく露時間: continuous for 1 h, 2 h or 3 h
GSM 2
ばく露3: 1,947.4 MHz
ばく露時間: continuous for 1 h, 2 h or 3 h
ばく露4: 915 MHz
ばく露時間: continuous for 1 h/day, 5 days/week, for 2 weeks
GSM 1
ばく露5: 905 MHz
ばく露時間: continuous for 1 h/day, 5 days/week, for 2 weeks
GSM 2
ばく露6: 1,947.4 MHz
ばく露時間: continuous for 1 h/day, 5 days/week, for 2 weeks

ばく露1

主たる特性
周波数 915 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 1 h, 2 h or 3 h
Additional information GSM 1
ばく露装置
ばく露の発生源／構造
Distance between exposed object and exposure source 1 m
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
電力 0.25 W - - - -
SAR 37 mW/kg - - - -

ばく露2

主たる特性
周波数 905 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 1 h, 2 h or 3 h
Additional information GSM 2
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
Distance between exposed object and exposure source 1 m
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
電力 0.25 W - - - -
SAR 37 mW/kg - - - -

ばく露3

主たる特性
周波数 1,947.4 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 1 h, 2 h or 3 h
Additional information UMTS
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
Distance between exposed object and exposure source 1 m
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
電力 0.25 W - - - -
SAR 39 mW/kg - - - -

ばく露4

主たる特性
周波数 915 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 1 h/day, 5 days/week, for 2 weeks
Additional information GSM 1
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
Distance between exposed object and exposure source 1 m
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
電力 0.25 W - - - -
SAR 37 mW/kg - - - -

ばく露5

主たる特性
周波数 905 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 1 h/day, 5 days/week, for 2 weeks
Additional information GSM 2
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
Distance between exposed object and exposure source 1 m
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
電力 0.25 W - - - -
SAR 37 mW/kg - - - -

ばく露6

主たる特性
周波数 1,947.4 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 1 h/day, 5 days/week, for 2 weeks
Additional information UMTS
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
Distance between exposed object and exposure source 1 m
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
電力 0.25 W - - - -
SAR 39 mW/kg - - - -

Reference articles

  • Sarimov R et al. (2004): [ヒトリンパ球でのクロマチン転座へのGSMマイクロ波の非熱的影響と熱ショックの類似性]
  • Weisbrot D et al. (2003): [キイロョウジョウバエの生殖および発生に対する携帯電話放射の影響]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

The data showed that microwave exposure inhibited the formation of 53BP1 foci in human primary fibroblasts and mesenchymal stem cells. The GSM carrier frequency (915 MHz) and UMTS frequency band (1947.4 MHz) were effective for both cell types. Exposure at 905 MHz did not inhibit 53BP1 foci formation in differentiated cells, either fibroblasts or lymphocytes (as found in a previous study, see Belyaev et al. 2009), whereas some effects were seen in stem cells at 905 MHz (statistically not significant trend to foci inhibition). Contrary to fibroblasts, stem cells did not adapt to chronic exposure during two weeks (i.e. foci inhibition was still present).
In conclusion, the data indicate that stem cells are more sensitive to microwave exposure than differentiated human primary fibroblasts (or lymphocytes as shown in the previous study). The fact that stem cells are more sensitive to microwave exposure and react to more frequencies than do differentiated cells may be important for cancer risk assessment and indicate that stem cells are the most relevant cellular model for validating safe mobile communication signals.

研究の種別:

研究助成

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