この研究は、細胞膜レベルでのマイクロ波相互作用の可能性を示す知見（単一チャネルおよび全電流記録によるパッチクランプ法により、低レベルのマイクロ波電磁界がニワトリ筋管初代培養におけるニコチン性アセチルコリン受容体の機能パラメータを変化させる可能性が示された）を踏まえ、このような影響への細胞内シグナル伝達プロセスの関与の可能性を調べた。L6-C5筋原細胞に、低電力電磁界ばく露を与えた後、ホスホリパーゼCおよびDのホルモン活性化への影響を観察した。その結果、アセチルコリンおよびアルギニンバソプレシンによるホスホリパーゼCの活性化によって誘導されるイノシトールリン脂質代謝回転の増加は、マイクロ波ばく露を受けた筋芽細胞または筋管で観察されなかった；また、バソプレッシン依存性のホスホリパーゼD活性化は、[(3)H]フリーコリン放出測定値で評価したが、これにもマイクロ波ばく露による変化は見られなかった；これらの知見は、低レベルのマイクロ波電磁界はL6-C5筋原性細胞のアセチルコリンとバソプレシンによって活性化されるシグナル伝達経路に変化を及ぼさないこと示唆する、と報告している。
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Ion channel proteins are possible sites of microwave interaction at the cell membrane level. Previous patch-clamp data indicated that low level microwave fields may alter some functional parameters of the nicotinic acetylcholine receptor in primary chick myotubes (publication 175) suggesting a possible effect of microwaves on myogenic cells.
The aim of this study was to investigate the biological relevance of such results, in relation to the possible involvement of intracellular signaling processes, i.e. it should be investigated whether microwave effects on acetylcholine receptor determine modifications to intracellular signalling.
Experiments were performed with proliferating cells (myoblasts) or differentiated cells (myotubes). Consequences on hormonal activation of phopholipase C and phopholipase D were observed (note: acetylcholine induces phosphoinositide breakdown by acting on the acetylcholine receptor and generating inositol phosphates and diacylglycerol; phopholipase C and D can act on diacylglycerol).
SAR value was estimated to be 64 mW/kg in the sample for the maximum applied power of 40 mW
| 周波数 | 10.75 GHz |
|---|---|
| ばく露時間 | 20 min, 40 min or 60 min |
| Modulation type | CW |
|---|
| ばく露の発生源／構造 | |
|---|---|
| Distance between exposed object and exposure source | 0.25 m |
| チャンバの詳細 | Incubator maintained at 37°C and contained 5% CO2; the internal part of the incubator was a metallic box which was divided into two compartments (50 cm x 45 cm) |
| ばく露装置の詳細 | Petri dishes, exposed one at a time, were placed in the upper compartment with the antenna inclined at 30° with respect to floor of the upper compartment and the E-vector was perpendicular to the incident plane |
| Additional information | The lower compartment of the incubator was shielded by metallic shelves to prevent exposure of control samples. |
| 測定量 | 値 | 種別 | Method | Mass | 備考 |
|---|---|---|---|---|---|
| 電力密度 | 4.5 µW/cm² | unspecified | 計算値 | - | - |
Increased inositol phospholipid turnover, induced by hormonal activation of phospholipase C, was not modified in microwave irradiated myoblasts or myotubes. Moreover, phospholipase D activation, assessed by measuring the [³H]-free choline release, was not modified by microwave exposure. The authors concluded that low level microwave fields do not modify signal transduction pathways activated by acetylcholine and vasopressin in L6-C5 myogenic cells.
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