Ion channel proteins are possible sites of microwave interaction at the cell membrane level. Previous patch-clamp data indicated that low level microwave fields may alter some functional parameters of the nicotinic acetylcholine receptor in primary chick myotubes (publication 175) suggesting a possible effect of microwaves on myogenic cells.
The aim of this study was to investigate the biological relevance of such results, in relation to the possible involvement of intracellular signaling processes, i.e. it should be investigated whether microwave effects on acetylcholine receptor determine modifications to intracellular signalling.
Experiments were performed with proliferating cells (myoblasts) or differentiated cells (myotubes). Consequences on hormonal activation of phopholipase C and phopholipase D were observed (note: acetylcholine induces phosphoinositide breakdown by acting on the acetylcholine receptor and generating inositol phosphates and diacylglycerol; phopholipase C and D can act on diacylglycerol).
| Exposure | Parameters |
|---|---|
|
Exposure 1:
10.75 GHz
Modulation type:
CW
Exposure duration:
20 min, 40 min or 60 min
|
|
|
Exposure 2:
10.75 GHz
Modulation type:
CW
Exposure duration:
20 min, 40 min or 60 min
|
|
SAR value was estimated to be 64 mW/kg in the sample for the maximum applied power of 40 mW
| Frequency | 10.75 GHz |
|---|---|
| Exposure duration | 20 min, 40 min or 60 min |
| Modulation type | CW |
|---|
| Exposure source | |
|---|---|
| Distance between exposed object and exposure source | 0.25 m |
| Chamber | Incubator maintained at 37°C and contained 5% CO2; the internal part of the incubator was a metallic box which was divided into two compartments (50 cm x 45 cm) |
| Setup | Petri dishes, exposed one at a time, were placed in the upper compartment with the antenna inclined at 30° with respect to floor of the upper compartment and the E-vector was perpendicular to the incident plane |
| Additional info | The lower compartment of the incubator was shielded by metallic shelves to prevent exposure of control samples. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| power density | 4.5 µW/cm² | unspecified | calculated | - | - |
| Frequency | 10.75 GHz |
|---|---|
| Exposure duration | 20 min, 40 min or 60 min |
| Modulation type | CW |
|---|
| Exposure source |
|
|---|---|
| Distance between exposed object and exposure source | 0.25 m |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| power density | 20 µW/cm² | unspecified | calculated | - | - |
Increased inositol phospholipid turnover, induced by hormonal activation of phospholipase C, was not modified in microwave irradiated myoblasts or myotubes. Moreover, phospholipase D activation, assessed by measuring the [³H]-free choline release, was not modified by microwave exposure. The authors concluded that low level microwave fields do not modify signal transduction pathways activated by acetylcholine and vasopressin in L6-C5 myogenic cells.
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