The system was allowed to equilibrate for 1 h prior to energizing the waveguides. The temperature within the cell cultures was monitored every 60 s and maintained within 37.0 ± 0.5°C for the sham, negative (incubator) control and RF-exposed samples. The samples exposed at an SAR of 10 W/kg showed cyclical fluctuations of ±0.2°C.
Concurrent negative and positive controls were included in each experiment. The positive (heat-shock) controls were placed on a heating block within an incubator and were maintained at 43°C for 1 h. The negative controls were placed within the same incubator but were not subjected to the heat shock.
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