EMF-Portal | Glioma proliferation modulated in vitro by isothermal radiofrequency radiation exposure

Glioma proliferation modulated in vitro by isothermal radiofrequency radiation exposure med./bio.

Aim of study (acc. to author)

Endpoint

Exposure

Exposure	Parameters
Exposure 1: 27 MHz Modulation type: CW Exposure duration: continuous for 2 h	SAR: 200 mW/g maximum (unspecified) SAR: 5 mW/g minimum (unspecified) (25 W/kg, 50 W/kg, 80 W/kg.)
Exposure 2: 2.45 GHz Modulation type: CW Exposure duration: continuous for 2 h	SAR: 5 mW/g minimum (unspecified) (25 W/kg, 50 W/kg.) SAR: 74.4 mW/g maximum (unspecified)

General information

Cell suspensions were exposed or sham exposed for 2 h.

Exposure 1

Main characteristics

Frequency	27 MHz
Type	electromagnetic field
Charakteristic	guided field
Exposure duration	continuous for 2 h

Modulation

Modulation type	CW

Exposure setup

Exposure source	waveguide

Parameters

Measurand	Value	Type	Method	Mass	Remarks
SAR	200 mW/g	maximum	unspecified	unspecified	-
SAR	5 mW/g	minimum	unspecified	unspecified	25 W/kg, 50 W/kg, 80 W/kg.

Exposure 2

Main characteristics

Frequency	2.45 GHz
Type	electromagnetic field
Charakteristic	guided field
Exposure duration	continuous for 2 h

Modulation

Modulation type	CW

Exposure setup

Exposure source	waveguide

Parameters

Measurand	Value	Type	Method	Mass	Remarks
SAR	5 mW/g	minimum	unspecified	unspecified	25 W/kg, 50 W/kg.
SAR	74.4 mW/g	maximum	unspecified	unspecified	-

Reference articles

Liu LM et al. (1988): Effects of 2.45-GHz microwave and 100-MHz radiofrequency radiation on liposome permeability at the phase transition temperature
Cleary SF et al. (1985): Erythrocyte hemolysis by radiofrequency fields

Methods Endpoint/measurement parameters/methodology

Investigated system:

DNA/RNA
intact cell/cell culture

Time of investigation:

after exposure

Main outcome of study (acc. to author)

Isothermal exposure of glioma cells in vitro modulated the rates of DNA and RNA synthesis 1, 3, and 5 days after exposure. The alterations indicate effects on cell proliferation and were not caused by radiofrequency-induced cell heating. The dose response for either frequency of the radiation was biphasic: Exposure to SARs of 50 W/kg or less stimulated incorporation rates of [³H]thymidine and [³H]uridine, whereas higher SARs suppressed DNA and RNA synthesis. Statistically significant time-dependent alterations were detected for up to 5 days postexposure, suggesting a kinetic cellular response to radiofrequency exposure and the possibility of cumulative effects on cell proliferation.

Study character:

medical/biological study
experimental study
full/main study

Study funded by

National Institutes of Health (NIH), Maryland, USA

Office of Naval Research (ONR), USA

Naziroglu M et al. (2012): 2.45-Gz wireless devices induce oxidative stress and proliferation through cytosolic Ca(2+) influx in human leukemia cancer cells

Trillo MA et al. (2011): Cytostatic response of NB69 cells to weak pulse-modulated 2.2 GHz radar-like signals

Hoyto A et al. (2008): Radiofrequency radiation does not significantly affect ornithine decarboxylase activity, proliferation, or caspase-3 activity of fibroblasts in different physiological conditions

Chauhan V et al. (2007): Evaluating the biological effects of intermittent 1.9 GHz pulse-modulated radiofrequency fields in a series of human-derived cell lines

Takashima Y et al. (2006): Effects of continuous and intermittent exposure to RF fields with a wide range of SARs on cell growth, survival, and cell cycle distribution

Glioma proliferation modulated in vitro by isothermal radiofrequency radiation exposure med./bio.

Aim of study (acc. to author)

Endpoint

Exposure

General information

Exposure 1

Main characteristics

Modulation

Exposure setup

Parameters

Exposure 2

Main characteristics

Modulation

Exposure setup

Parameters

Reference articles

Methods Endpoint/measurement parameters/methodology

Main outcome of study (acc. to author)

Study funded by

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