Study type:
Medical/biological study
(experimental study)
In vitro exposure of human lymphocytes to 900 MHz CW and GSM modulated radiofrequency: studies of proliferation, apoptosis and mitochondrial membrane potential
med./bio.
By:
Capri M, Scarcella E, Fumelli C, Bianchi E, Salvioli S, Mesirca P, Agostini C, Antolini A, Schiavoni A, Castellani G, Bersani F, Franceschi C
The TEM cell (107 x 51 x 51 cm, septum height 23 cm) was positioned vertically inside a thermostatic room maintained at 37°C.
Setup
Microplates were located in the middle section of the cell on the right and on the left side of the septum on a thin plastic shelf (2 mm).
Additional info
A 96 well microplate (127 x 84 x 15 mm) or two 24 well microplates (127 x 84 x 22 mm) placed one upon the other were used. Well diameters and volume of culture medium were 6.4 or 16 mm and 200 µl or 2 ml, respectively.
The first hour of exposure took place immediately after cell seeding and treatment with mitogenic or apoptosis inducers, the second and third hours took place after 24 or 48 h of culture.
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In vitro effect of pulsed 900 MHz GSM radiation on mitochondrial membrane potential and motility of human spermatozoa
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Palumbo R et al.
(2008):
Exposure to 900 MHz radiofrequency radiation induces caspase 3 activation in proliferating human lymphocytes
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Apoptosis is Induced by Radiofrequency Fields through the Caspase-Independent Mitochondrial Pathway in Cortical Neurons
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(2007):
Evaluating the biological effects of intermittent 1.9 GHz pulse-modulated radiofrequency fields in a series of human-derived cell lines
Joubert V et al.
(2007):
No apoptosis is induced in rat cortical neurons exposed to GSM phone fields
Hirose H et al.
(2006):
Phosphorylation and gene expression of p53 are not affected in human cells exposed to 2.1425 GHz band CW or W-CDMA modulated radiation allocated to mobile radio base stations
Lantow M et al.
(2006):
Comparative study of cell cycle kinetics and induction of apoptosis or necrosis after exposure of human mono mac 6 cells to radiofrequency radiation
Gurisik E et al.
(2006):
An in vitro study of the effects of exposure to a GSM signal in two human cell lines: monocytic U937 and neuroblastoma SK-N-SH
Pavicic I et al.
(2006):
Comparison of 864 MHz and 935 MHz microwave radiation effects on cell culture