The study was designed to determine in human peripheral blood lymphocytes: 1) If continuous wave (CW) radiofrequency radiation (RF) directly affects lymphocytes independently of radiation-induced heating; 2) dose-response relationships for radiofrequency-induced alterations; and 3) relative effects of two RF frequencies (27 and 2450 MHz).
Immediately after exposure, mononuclear cells were separated from blood by density-gradient centrifugation and cultured for 3 days at 37°C with or without mitogenic stimulation by phytohemagglutinin (PHA).
| Exposure | Parameters |
|---|---|
|
Exposure 1:
27 MHz
Modulation type:
CW
Exposure duration:
2 h
|
|
|
Exposure 2:
2.45 GHz
Modulation type:
CW
Exposure duration:
2 h
|
|
| Frequency | 27 MHz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | 2 h |
| Modulation type | CW |
|---|
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| SAR | 5 mW/g | unspecified | unspecified | unspecified | - |
| SAR | 196 mW/g | unspecified | unspecified | unspecified | 814 V/m corresponding electric field |
| SAR | 50 mW/g | unspecified | unspecified | unspecified | 208 V/m corresponding electric field |
| SAR | 25 mW/g | unspecified | unspecified | unspecified | 104 V/m corresponding electric field |
| Frequency | 2.45 GHz |
|---|---|
| Type | |
| Waveform | |
| Exposure duration | 2 h |
| Modulation type | CW |
|---|
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| SAR | 5 mW/g | unspecified | unspecified | unspecified | - |
| SAR | 50 mW/g | unspecified | unspecified | unspecified | 145 V/m corresponding electric field |
| SAR | 25 mW/g | unspecified | unspecified | unspecified | 72.5 V/m corresponding electric field |
| SAR | 39.5 mW/g | unspecified | unspecified | unspecified | 114.5 V/m corresponding electric field |
The results indicate a direct, athermal effect of radiofrequency radiation on human lymphocyte proliferation. A single 2-h radiation at either frequency caused a consistent, statistically significant, dose-dependent biphasic modulation of lymphocyte proliferation in both phytohemagglutinin (PHA) stimulated and unstimulated cultures. Exposure at either frequency at specific absorption rates (SARs) below 50 W/kg resulted in a dose-dependent, statistically significant increase of [³H]-thymidine uptake in PHA-activated or unstimulated lymphocytes. Exposure at 50 W/kg or higher suppressed [³H]-thymidine uptake relative to that of sham-exposed cells. There were no detectable effects of radiofrequency exposure on lymphocyte morphology or viability.
This website uses cookies to provide you the best browsing experience. By continuing to use this website you accept our use of cookies.
