Electromagnetic fields affect transcript levels of apoptosis-related genes in embryonic stem cell-derived neural progenitor cells med./bio.

Aim of study (acc. to editor)

Background/further details

Endpoint

• different effects (e.g. different transcript levels, genotoxicity, proliferation, apoptosis, cytotoxicity, mitochondrial function)

Exposure

• • 50/60 Hz
• • power transmission line
  • mobile communications
  • GSM

Extended view Compact view

Methods Endpoint/measurement parameters/methodology

• genotoxicity/mutation: single-strand breaks, double-strand breaks (alkaline and neutral comet assay); chromosomal aberrations, sister chromatid exchanges (differentially stained by FPG technique)
• molecular biosynthesis: mRNA-levels/transcript levels of cell cycle regulatory (GADD45), apoptosis-related (bcl-2, bax), and neural-specific (Nurr1) genes (and of p53, TH, and nestin; RT-PCR)
• cell function: mitochondrial function (mitochondrion-selective stain (Mitotacker CM-H2X ROS))
• cell viability/cell division/proliferation: cell proliferation (BrdU incorporation); quantification of apoptosis (content of hypodiploid DNA; flow cytometry)

Main outcome of study (acc. to author)

Study funded by

• European Union (EU)/European Commission
• Fonds der chemischen Industrie (FCI; Foundation of the Chemical Industry), Germany
• VERUM Foundation (Stiftung für Verhalten und Umwelt), Germany

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