Study type: Medical/biological study (experimental study)

Immunotropic effects in cultured human blood mononuclear cells pre-exposed to low-level 1300 MHz pulse-modulated microwave field med./bio.

By: Dabrowski MP, Stankiewicz W, Kubacki R, Sobiczewska E, Smigielski S
Published in: Electromagn Biol Med 2003; 22 (1): 1-13
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Aim of study (acc. to author)

To determine potential immunomodulatory influences of pulse-modulated 1300 MHz microwave field on blood mononuclear cells (G0 phase cells) isolated from 16 healthy donors.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 1,300 MHz
Modulation type: pulsed
Exposure duration: continuous for 1 h

Exposure 1

Main characteristics
Frequency 1,300 MHz
Type
Charakteristic
Polarization
Exposure duration continuous for 1 h
Additional info vertically polarised
Modulation
Modulation type pulsed
Pulse width 5 µs
Repetition frequency 330 Hz
Exposure setup
Exposure source
Distance between exposed object and exposure source 1.5 m
Chamber 5.5 m long x 2.2 m wide x 2.1 m high
Setup A specimen of 3 ml of isolated PBMC was placed in a sterile tube.
Parameters
Measurand Value Type Method Mass Remarks
power density 6,000 W/m² peak value measured - -
power density 10 W/m² mean measured - -
electric field strength 61.4 V/m mean measured - -
SAR 0.18 W/kg mean determined by power loss - -
Measurement and calculation details

Pulse parameters and waveform were determined using oscilloscope and frequency meter coupled to the waveguide. The incident field at the exposure point was measured in absence of the sample using a NARDA meter. The SAR of the sample was determined by power loss measurement in a rectangular waveguide WR 650 [Chou et al., 1984; Guy et al., 1979].

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The results indicate that the exposure of the cells influenced the monocyte-dependent immunoregulatory mechanisms responsible for the initiation of immune response. The exposure further significantly increased the production of IL-1 and decreased the production of IL-1ra. The changed ratio of IL-1/IL-1ra resulted in an increased value of lymphocyte-monocyte cooperation index, which reflects the activation of immunogenic and proinflammatory functions of monocytes. The proliferative response of T lymphocytes to mitogens (PHA, concanavalin A) was not changed. But the exposure significantly increased the production of IL-10, the immunoregulatory lymphokine that is involved in the T cell-monocyte interaction. The immunstimulatory effects of the 1300 MHz fields seem to affect preferentially the immunogenic functions of monocytes and the increased production of IL-10 seems to be a secondary reaction of T cells in response to activated monocytes.

Study character:

Study funded by

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