In a previous study (publication 3775), it was shown that exposure of transformed human epithelial amnion cells (AMA) to a modulated radiofrequency field at 960 MHz at different power levels and exposure times, resulted in significant changes in cell proliferation.
In this study, the cells, the radiofrequency field, and the exposure time were the same, but the cells were exposed at a higher or lower temperature (39 or 35 +/- 0.1°C).
| Exposure | Parameters |
|---|---|
|
Exposure 1:
960 MHz
Modulation type:
pulsed
Exposure duration:
continuous for 30 min
|
|
| Frequency | 960 MHz |
|---|---|
| Type | |
| Exposure duration | continuous for 30 min |
| Exposure source | |
|---|---|
| Chamber | incubator |
| Setup | The cells were seeded into the two central rows (#6 and #7) of sterile 96-well microtiter plates, each well containing a volume of 100 µl. One plate was placed in the TEM cell (powered or not) while the control plate was placed 20 cm away from it. |
| Additional info | Static and AC (50 Hz) magnetic fields, measured with a three-axis fluxgate meter in both incubators did not exceed 1 µT. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| electric field strength | 17 mV/cm | peak value | calculated | - | - |
| SAR | 2.1 µW/g | - | calculated | - | in the cell culture |
There was at both temperatures a significant change in cell proliferation in the exposed cells in comparison to the control cells. On the other hand no significant change in proliferation rate was found in the sham-exposed cells at both temperatures. This shows that biological effects due to radiofrequency cannot be attributed only to a change of temperature.
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