この研究は、一連の6つの円偏波円筒導波管を使用して、ヒトの血液培養物に1.9 GHzの連続波（CW）無線周波（RF）電磁界へのばく露を2時間与えた。平均SARは、0.0、0.1、0.26、0.92、2.4、および10 W/ kgとし、2時間のばく露中の培養物内の温度は37.0±0.5 ℃に維持された。 同様にインキュベートした無ばく露対照群、ガンマ線照射（1.5 Gy）した陽性対照群を実験ごとに用いた。アルカリコメットアッセイを用いて、ばく露終了直後にDNA損傷を定量化し、4つのパラメータ（テール比、テールモーメント、コメット長、テール長）を用いてDNA損傷を評価した。また、細胞質分裂ブロック小核アッセイを用いて、小核形成を評価した。その結果、どのSARレベルのばく露を受けた培養物でも、どのコメットパラメータによっても、一次DNA損傷の増加は示されなかった；どのSARでのばく露培養とその擬似ばく露対照との間にも、二核細胞の頻度、小核二核細胞の発生頻度、小核の総発生頻度に有意差はなかった、と報告している。
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To investigate whether acute (2h) exposure to a 1.9 GHz continuous wave (CW) radiofrequency field could elicit primary DNA damage and/or induce micronucleus formation in cultured human leukocytes.
| 周波数 | 1.9 GHz |
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| タイプ |
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| 特性 |
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| 偏波 |
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| ばく露時間 | continuous for 2 h |
| Modulation type | CW |
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| ばく露の発生源／構造 |
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| チャンバの詳細 | The waveguide applicators consisted of an aluminium tube, 110 mm in diameter and 210 mm long, which was shorted at one end and open at the other. They were fed from two identical excitation probes (monopoles, 33 mm long x 4 mm in diameter) spaced 90° apart and located 50 mm from the shorted end. All six waveguide applicators were housed in a wooden enclosure lined with MW absorbent foam. They were spaced 250 mm apart (center to center) and were separated by a shield consisting of a 200 mm x 300 mm aluminium sheet sandwiched between 6.4-mm thick absorber panels and extending 100 mm higher than the top of the sample dishes. |
| ばく露装置の詳細 | The sample holder located on the top of the open aperture of the waveguide consisted of two unequal-sized Petri dishes. The inner dish of 60 mm diameter containing the blood cell culture sat concentrically within the outer dish of 150 mm diameter on 3.2-mm plastic standoffs and was circulated by temperature-controlled coolant water maintaining the temperature in the culture at 37 ± 0.5°C. The coolant (130 ml) was kept at the same level as the sample (10 ml) for optimal SAR uniformity. |
| Sham exposure | A sham exposure was conducted. |
| Additional information | A total of five separate experiments were conducted over 3 months, each consisting of five RF-field exposed cultures and concurrent sham exposed, negative (incubator) and positive (1.5 Gy 137Cs γ radiation; at 0.987 Gy/min) controls. |
The results provide no evidence to support the hypothesis that acute, nonthermal, RF-field exposure causes appreciable DNA damage in human leukocytes.
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