研究のタイプ: 医学／生物学の研究 (experimental study)

[イン・ビトロでセルラー電話周波数（835.62MHz；FDMA）の無線周波放射にばく露したヒト血液リンパ球における細胞遺伝学的研究] med./bio.

Cytogenetic studies in human blood lymphocytes exposed in vitro to radiofrequency radiation at a cellular telephone frequency (835.62 MHz, FDMA)

著者: Vijayalaxmi, Leal BZ, Meltz ML, Pickard WF, Bisht KS, Roti Roti JL, Straube WL, Moros EG
掲載誌: Radiat Res 2001; 155 (1): 113-121
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この研究は、４人の健康なヒトボランティアからの新しく採取した末梢血試料を培養液で希釈してフラスコに入れ、24時間、835.62 MHzのRF電磁界ばく露を与え、細胞遺伝学的損傷の有無を調べた。ばく露に用いたＦＤＭＡの835.62 MHzは、公称正味前方電力68 W、ばく露を受けたフラスコ中心での公称電力密度は860 W/m2、フラスコ内の平均比吸収率は4.4 または5.0 W/kgであった。希釈された採取血液のアリコート（全体から取り出した一部の試料）に、擬似ばく露または1.50 Gyガンマ線照射を与えたものを陰性または陽性対照として使用した。いずれもばく露直後にリンパ球をマイトジェン（有糸分裂促進因子）で刺激し、48または72時間培養した後、染色体異常および小核の頻度を評価して、遺伝的損傷の大きさを決定した。細胞増殖の動力学的変化の大きさは、48時間培養における有糸分裂指数および72時間培養における二核細胞の発生率から決定された。その結果、有糸分裂指数、染色体の交換異常発生率、過剰な断片、二核細胞、および小核に関して、RFばく露リンパ球と擬似ばく露リンパ球との間に有意差はなかった；対照的に、ガンマ線ばく露リンパ球の反応は、これら全ての指標について、RFばく露および擬似ばく露リンパ球のどちらとも有意差があった、と報告している。

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研究目的(acc. to editor)

Aim of the study was to investigate the extent of possible genetic damage caused by radiofrequency irradiation (835.62 MHz, 24 h, FDMA) on human blood lymphocytes in vitro.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 835.62 MHz
Modulation type: CW
ばく露時間: continuous for 24 h
  • 電力: 68 W (net forward power)
  • 電力密度: 860 W/m² (at the center of the flask)
  • SAR: 4.4 W/kg mean
  • SAR: 5 W/kg mean

ばく露1

主たる特性
周波数 835.62 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 24 h
Modulation
Modulation type CW
Additional information

FDMA/CW
ばく露装置
ばく露の発生源／構造
チャンバの詳細 The facility housed nine RTLs (radial transmission lines) mounted on two separate adjacent sliding shelves. The facility and RTLs were shielded to avoid interference. Each RTL was formed by two parallel horizontal metal plates 4.3 cm apart, with a lightweight composite top plate hinged to the bottom aluminium plate (0.6 cm thick) providing mechanical support and homogeneity of the flasks' temperatures. The waves travelling from the central conical antenna were terminated by an annulus of MW-absorbing foam backed by perforated aluminium. The total radius was 53.3 cm.
ばく露装置の詳細 Each RTL was fully loaded with 16 T-75 culture flasks at a radius of 29.2 cm each containing 40 ml of liquid medium (about 5 mm depth) (two of them diluted blood). The distance of the cells settled at the bottom of the flask from the antenna was between 24.7 cm and 33.8 cm.
Additional information Aliquots of blood that were sham-exposed or exposed to 1.5 Gy of γ radiation were used as negative or positive controls.
パラメータ
測定量 値 種別 Method Mass 備考
電力 68 W - - - net forward power
電力密度 860 W/m² - - - at the center of the flask
SAR 4.4 W/kg mean 測定値および計算値 - -
SAR 5 W/kg mean 測定値および計算値 - -
測定および計算の詳細

The SARs of flasks were measured by the temperature rise method using both a thermographic and a thermometric method. The experimental results were in reasonable agreement with those obtained from a three-dimensional FDTD numerical model analysis.

Reference articles

  • Moros EG et al. (1999): [大量の培養瓶へのマイクロ波照射用広帯域遮蔽ばく露装置としての放射状送電線]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

The overall cytogenetic responses of the lymphocytes were similar although the absolute values were slightly different. The mitotic indices, the indices of abnormal cells showing chromosomal damage, and the numbers of exchange aberrations were not significantly different between radiofrequency irradiation and sham-exposed cells. Similarly, the percentages of binucleated cells, the incidence of binucleated cells with one, two or three micronuclei, and the total numbers of micronuclei were not significantly different.

研究の種別:

研究助成

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