研究のタイプ: 医学／生物学の研究 (experimental study)

[原生動物のコンピュータによる生体形態計測：携帯電話電磁界ばく露中の細胞における形態機能的障害の監視方法] med./bio.

Intravital Computer Morphometry on Protozoa: A Method for Monitoring of the Morphofunctional Disorders in Cells Exposed in the Cell Phone Communication Electromagnetic Field

著者: Uskalova DV, Igolkina YV, Sarapultseva EI
掲載誌: Bull Exp Biol Med 2016; 161 (4): 554-557
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この研究は、周波数1GHz、電力密度50μW/cm2の電磁界への30、60、360分間のばく露後の単細胞水生原虫の形態機能的障害を、コンピュータによる生体形態計測で調べた。その結果、形態計測値における有意な障害は、原生生物の移動性の低さと相関していた。著者らは、この結果は、モバイル通信用の電磁界による影響（例えば原虫の移動性の低さ）についての早期診断のための、原生生物に対するコンピュータによる生体形態計測の利用を示唆している、と結論付けている。

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研究目的（著者による）

The effects of exposure of protozoa Spirostomum ambiguum to a 1 GHz electromagnetic field on some morphometric parameters should be investigated.

詳細情報

Spirostomum ambiguum is a common test organism in ecotoxicology and was already used in former studies by the authors (Sarapultseva et al. (2009), Sarapultseva and Igolkina (2011), Sarapultseva et al. (2014)).
Cells were divided into the following groups: exposure to the electromagnetic field for 1) 30, 2) 60 and 3) 360 min and 4) sham exposure. Morphometry was carried out on the day after exposure.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1 GHz
ばく露時間: continuous for 30, 60 and 360 minutes

ばく露1

主たる特性
周波数 1 GHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 30, 60 and 360 minutes
ばく露装置
ばく露の発生源／構造
  • 詳細不明
チャンバの詳細 open petri dishes (3.5 cm in diameter)
ばく露装置の詳細 30-50 cells/ml in 4 ml water were exposed in the exposure device at 20-22°C ± 0.1°C
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
電力密度 50 µW/cm² - 測定値 - -
Additional parameter details

background electromagnetic field power flux density in the laboratory was not more than 1.5 µW/cm2

測定および計算の詳細

power flux density was measured with a device with 12% error of measurements

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

All morphometric parameters were significantly decreased in all exposure groups (group 1-3) compared to the sham exposure group, indicating a significant change of cell morphology.
These morphometric changes correlated (remark EMF-Portal: no details on correlation given) with a lower mobility of Spirostomum ambiguum exposed according to a similar protocol in a former study by the authors (Sarapultseva et al. (2014)).
The authors conclude that exposure of protozoa Spirostomum ambiguum to a 1 GHz electromagnetic field might change some morphometric parameters. They assume their morphometric method might be feasible in early diagnosis of effects of electromagnetic fields on cells.

研究の種別:

研究助成

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