The effects of exposure of protozoa Spirostomum ambiguum to a 1 GHz electromagnetic field on some morphometric parameters should be investigated.
Spirostomum ambiguum is a common test organism in ecotoxicology and was already used in former studies by the authors (Sarapultseva et al. (2009), Sarapultseva and Igolkina (2011), Sarapultseva et al. (2014)).
Cells were divided into the following groups: exposure to the electromagnetic field for 1) 30, 2) 60 and 3) 360 min and 4) sham exposure. Morphometry was carried out on the day after exposure.
| Exposure | Parameters |
|---|---|
|
Exposure 1:
1 GHz
Exposure duration:
continuous for 30, 60 and 360 minutes
|
|
| Frequency | 1 GHz |
|---|---|
| Type | |
| Exposure duration | continuous for 30, 60 and 360 minutes |
| Exposure source |
|
|---|---|
| Chamber | open petri dishes (3.5 cm in diameter) |
| Setup | 30-50 cells/ml in 4 ml water were exposed in the exposure device at 20-22°C ± 0.1°C |
| Sham exposure | A sham exposure was conducted. |
| Measurand | Value | Type | Method | Mass | Remarks |
|---|---|---|---|---|---|
| power density | 50 µW/cm² | - | measured | - | - |
All morphometric parameters were significantly decreased in all exposure groups (group 1-3) compared to the sham exposure group, indicating a significant change of cell morphology.
These morphometric changes correlated (remark EMF-Portal: no details on correlation given) with a lower mobility of Spirostomum ambiguum exposed according to a similar protocol in a former study by the authors (Sarapultseva et al. (2014)).
The authors conclude that exposure of protozoa Spirostomum ambiguum to a 1 GHz electromagnetic field might change some morphometric parameters. They assume their morphometric method might be feasible in early diagnosis of effects of electromagnetic fields on cells.
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