この研究は、ヒト胎児強膜線維芽細胞（HFSFs）に対する超低周波磁界（ELF-MF）の影響を調べた。ヘルムホルツコイルを用いて発生させたELF-MF（50Hz、磁束密度0.1、0.2、0.5、1.0mT）のばく露を、ばく露時間6-48時間で実施した。影響は、生存率および強膜構造に関与する因子で評価した。その結果、0.2 mTの24時間ばく露で、HFSFsの増殖速度が有意に低下した；コラーゲンタイプIのメッセンジャRNA発現減少およびマトリックスメタロプロテアーゼ2の発現増加が有意であった､などの所見を報告している。
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To investigate the effects of extremely low frequency magnetic fields on cell growth and factors involved in scleral structuring in scleral fibroblasts.
The possible impact of extremely low frequency magnetic fields on pathological alterations in the sclera has been rarely documented. However, it might play an important role in treating certain eye ailments (e.g. myopia) and therefore more research is required.
The sclera is a part of the human eye ("white of the eye"), mainly composed of collagen fibrils. Several factors can influence the correct formation and structure of these collagen fibrils. Therefore, the expression of several genes related to the collagen synthesis was examined.
The cells were exposed to a magnetic flux densitiy of 0.2 mT for 24 hours if not stated otherwise.
| ばく露 | パラメータ |
|---|---|
|
ばく露1:
50 Hz
ばく露時間:
continuous for 24 hours
cell proliferation, protein expression, cell morphology
|
|
|
ばく露2:
50 Hz
ばく露時間:
continuous for 24 hours
gene expression experiment 1
|
|
|
ばく露3:
50 Hz
ばく露時間:
continuous for up to 48 hours (6, 12, 24, 36 or 48 hours)
gene expression experiment 2
|
|
| 周波数 | 50 Hz |
|---|---|
| タイプ |
|
| 波形 |
|
| ばく露時間 | continuous for 24 hours |
| Additional information | cell proliferation, protein expression, cell morphology |
| ばく露の発生源／構造 | |
|---|---|
| ばく露装置の詳細 | two exposure facilities (one for exposure, the other for sham exposure) with the same environment were put into two thermo-regulated and atmosphere-controlled incubators at 37°C and 5% CO2; the coils were 16 cm long, 14 cm wide and 8 cm high and consisted of 168 turns copper wire, respectively |
| Sham exposure | A sham exposure was conducted. |
| 測定量 | 値 | 種別 | Method | Mass | 備考 |
|---|---|---|---|---|---|
| 磁束密度 | 0.2 mT | - | 測定値 | - | - |
The cell proliferation of exposed cells was significantly decreased compared to the control group. Additionally, the morphology of exposed cells differed from those of the control group: While untreated cells appeared fibroblast-like (spindle or bundle shape), the alignment of exposed cells was altered into irregular polygonal configurations.
The gene expression of collagen 1 (starting after 6 hours) and fibroblast growth factor-2 (starting after 12 hours) was significantly decreased after exposure to 0.2 mT and higher magnetic flux densities in comparison to the control, while the gene expression of matrix-metalloproteinase-2 (starting after 24 hours; 0.1 and 0.2 mT) and transforming growth factor beta-2 (starting after 24 hours; 0.5 mT and 1 mT) was significantly increased. However, the gene expression level of the matrix-metalloproteinase-2 inhibitor was significantly increased only after exposure to 1 mT compared to the control.
The protein expression analysis was in agreement with the results of the gene expression examination: In exposed cells, the protein expression of collagen 1 was significantly decreased compared to the control while the protein expression of matrix-metalloproteinase-2 was significantly increased.
The authors conclude that extremely low frequency magnetic fields could influence the growth of scleral fibroblasts as well as its gene expression and protein expression related to the structure of the sclera.
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