この研究は、インビトロで繁殖中のコーヒーノキ（Coffea arabica L.）の苗木をソレノイドコイル（40cm）の内部に置き、60Hz、2mTの磁界ばく露の影響を調べた。活着期または増殖期の1週間後、あるいは移植後（順応期）に3分間の磁界ばく露を行った。50個の胚芽、50本の植物体での実験を3回繰り返した。その結果、それぞれの期での磁界ばく露群で、対照群に比べ、シュートおよび根の長さ、葉対の数が増加した､などを報告している。
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To investigate the possible effect of exposure to extremely low frequency magnetic fields on growth and antioxidative enzymes in in vitro (micropropagation) coffee seedlings and subsequent development of these seedlings.
Coffee is an important commercial product. Micrpropagation presents an in vitro culture system to generate high quality coffee seedlings. After a six weeks establishment phase in a culture medium, the plants reach a multiplication phase usually having three pairs of leaves and a primary root at the end. Afterwards, in the acclimatization phase, plants are transferred to a pot with soil.
Seedlings were exposed or sham exposed either during establishment, multiplication, or acclimatization for 3 minutes. For each experimental group (exposure and sham exposure, respectively), 50 samples were used per replicate and 3 replicates for all phases. Seedlings were examined at the end of the each phase or 4 month after the acclimatization phase,
| ばく露 | パラメータ |
|---|---|
|
ばく露1:
60 Hz
ばく露時間:
3 minutes
|
|
| 周波数 | 60 Hz |
|---|---|
| タイプ |
|
| ばく露時間 | 3 minutes |
| ばく露の発生源／構造 | |
|---|---|
| ばく露装置の詳細 | samples were placed in a dielectric container and placed in the homogeneous magnetic field at the end and center of the solenoid; a 40 cm long magnetizer solenoid was used, consisting of a 16-gauge copper wire (1.1 mm) with a total of 363 turns |
| 測定量 | 値 | 種別 | Method | Mass | 備考 |
|---|---|---|---|---|---|
| 磁束密度 | 2 mT | - | - | - | - |
In exposed seedlings, the shoot and root lengths as well as leaf pair numbers were significantly increased during establishment, multiplication, or acclimatization phase and 4 month after acclimatization in comparison to seedlings from the control group. Magnetic field treatment resulted in a significant decrease of the enzyme activity of the catalase during establishment, multiplication and acclimatization, of the superoxide dismutase during acclimatization and of the ascorbate peroxidase during multiplication, acclimatization and in the 4 month old seedling compared to the corresponding control group. However, the enzyme activity of the catalase was significantly increased in the exposed 4 month old seedlings compared to the 4 month old control seedlings.
The authors conclude that exposure to extremely low frequency magnetic fields could influence antioxidative enzymes in in vitro (micropropagation) coffee seedlings and could have a promoting effect on growth and development.
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