この研究は、末期肝不全治療のための高分子ナノファイバ足場と幹細胞を用いた肝組織工学への低周波磁界の利用を検討した。ポリL乳酸（PLLA）、コラーゲン、高濃度フィブリン血液から生体磁気的電界紡糸により製作されたナノファイバ足場上のヒト間葉系幹細胞（hMSCs）が、低周波磁界下で肝細胞に形質転換する潜在力を評価した。足場の表面特性、生化学的および生体機械学的パラメータ、生体磁気的ふるまいに関する特徴を記述している。その結果、用いた足場はhMSCsの付着および増殖に適していることが示された；磁界ばく露群の方が対照群に比べ、足場上のhMSCsの肝細胞への形質転換力が高まった､と報告している。
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The effects of exposure of human mesenchymal stem cells to a 50 Hz magnetic field grown on a nanofibrous scaffold on hepatic differentiation should be investigated. A possible therapeutic use for the treatment of hepatic diseases via tissue engineering should be examined.
Cell differentiation was induced by the addition of different growth factors.
Cells were divided into the following groups: 1) cells with growth factors exposed to the magnetic field and cultivated on nanofibrous scaffold, 2) non-exposed cells with growth factors cultivated on nanofibrous scaffold, 3) non-exposed cells with growth factors cultivated on polystyrenes scaffolds, 4) non-exposed cells without growth factors cultivated on nanofibrous scaffold, 5) non-exposed cells without growth factors cultivated on polystyrenes scaffold.
HepG2 cells were used as positive control in the test on the amount of inducible cytochrome P450.
All experiments were performed in triplicates.
| ばく露 | パラメータ |
|---|---|
|
ばく露1:
50 Hz
ばく露時間:
continuous for 28 days
|
|
| 周波数 | 50 Hz |
|---|---|
| タイプ |
|
| ばく露時間 | continuous for 28 days |
| ばく露の発生源／構造 | |
|---|---|
| チャンバの詳細 | culture flasks |
| ばく露装置の詳細 | a solenoid iron coil was placed inside an incubator with 5% CO2 and 37°C; the culture flasks were placed over the magnetic field generator |
| 測定量 | 値 | 種別 | Method | Mass | 備考 |
|---|---|---|---|---|---|
| 磁束密度 | 60 mT | - | - | - | - |
Morphologically, more polygonal shaped cells, representative for hepatic morphology, were found in group 1 (growth factors, MF, nanofibrous scaffold) than in group 2 (growth factors, no MF, nanofibrous scaffold) and group 3 (growth factors, no MF, polystyrenes scaffold).
Immunofluorescence staining confirmed these results by showing the highest protein expression of hepatocyte specific markers in group 1, a scanty protein expression in groups 2 and 3, and no protein expression in groups without growth factors (groups 4 and 5).
The amounts of albumin and urea were significantly increased in group 1 compared to groups 2 and 3 (groups 4 and 5 did not show any presence of albumin or urea) and likewise, group 1 showed the highest amount of inducible cytochrome P450.
The authors conclude that exposure of human mesenchymal stem cells to a 50 Hz magnetic field grown on the nanofibrous scaffold might augment the hepatic differentiation.
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