研究のタイプ: 医学／生物学の研究 (experimental study)

[無線周波ばく露後のマウスの上オリーブ複合体での脳由来およびグリア細胞株由来の神経栄養因子の免疫組織化学的局在] med./bio.

Immunohistochemical localization of brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor in the superior olivary complex of mice after radiofrequency exposure

著者: Maskey D, Kim MJ
掲載誌: Neurosci Lett 2014; 564: 78-82
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この研究は、無線周波（RF）ばく露を受けたICRマウス（n = 10）の上オリーブ複合体（SOC）標本における脳由来神経栄養因子（BDNF）およびグリア細胞株由来神経栄養因子（GDNF）の分布を免疫組織化学的に測定した。BDNFおよびGDNFは、蝸牛神経などの生存、聴覚損傷に関与するとされる。マウスは5匹ずつのばく露群、擬似ばく露群に分け、ばく露群は835 MHzにRF全身ばく露（SAR = 1.6 W/kg）した。ばく露（擬似ばく露）は、1日8時間、3ヶ月間継続した。その結果、ばく露群では、BDNFの免疫活性の有意な低下が外側上オリーブ核、内側上オリーブ核などの各所で見られた；同様に、GDNFの免疫活性の有意な低下がSOCの全ての核で見られた、と報告している。

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研究目的（著者による）

The effects of exposure of mice to an 835 MHz electromagnetic field on the protein expression of the brain-derived neurotrophic factor and glial-derived neurotrophic factor in the central auditory system of the brain should be investigated.

詳細情報

Given the close proximity between a mobile phone and the ear, it has been suggested that the central auditory system may be detrimentally influenced by radiofrequency exposure. The brain-derived neurotrophic factor and glial-derived neurotrophic factor are important growth factors for the maintenance of auditory neurons.
10 mice were divided into 2 groups (n=5 each): 1) exposure to the electromagnetic field and 2) sham exposure. 10 brain sections per animal were chosen randomly for analysis.
Negative control was performed.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 835 MHz
ばく露時間: continuous for 8 h/day for 3 months

ばく露1

主たる特性
周波数 835 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 8 h/day for 3 months
ばく露装置
ばく露の発生源／構造
チャンバの詳細 22 inches mouse cage; mice were able to move freely in cage; cage was surrounded by wave absorption material (TDK ceramic absorber)
ばく露装置の詳細 mouse cage was equipped with "Wave Exposer V20" composed of pyramidal horn antenna connected by a waveguide to coaxial transition
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 1.6 W/kg - 測定値および計算値 - -
電力 2.5 W - - - output power of antenna
電界強度 59.56 V/m - 計算値 - -

Reference articles

  • Maskey D et al. (2010): [マウスの脳の海馬におけるカルシウム結合タンパク質に対する835 MHz高周波放射へのばく露の影響]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

Protein expression of the brain-derived neurotrophic factor and glial-derived neurotrophic factor was significantly reduced in all examined brain areas in the exposure group compared to the sham exposure group.
The authors conclude that exposure of mice to an 835 MHz electromagnetic field could have an effect on the protein expression of the brain-derived neurotrophic factor and glial-derived neurotrophic factor in the central auditory system, which in turn might affect its function.

研究の種別:

研究助成

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