この研究は、正常マウスBリンパ球の原形質膜抗原-抗体複合体キャップ能力に対する2.45GHz連続波マイクロ波（電力密度100mW / cm 2；SARは45W / kg）ばく露の影響を調べた。ばく露（３０分間）は、環境温度37、41、42.5℃で行われた。ばく露処理後に、ばく露群および無ばく露対照群の細胞について、直接免疫蛍光法を用いてキャップされた細胞のパーセンテージを蛍光顕微鏡下で測定した。その結果、無ばく露対照群では、キャッピングが、37℃での90％から、41℃では52％に、42.5℃では5％未満に低下した；同じ温度条件下では、ばく露群と対照群の間に有意差はなかった；することを示している。マイクロ波処理した細胞およびコントロールを両方とも同じ温度に維持した場合に、別の実験では、両方の調製物の温度を38.5℃に保ったキャッピングの間に、マイクロ波照射に暴露された対照と細胞との間のキャッピングの割合に有意差はなかった、と報告されている。
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To study the combined in vitro effects of microwaves and hyperthermia on the ability of normal B lymphocytes to cap surface immunoglobulin (Ig).
Normal mouse B lymphocytes were tested following exposure to 2.45-GHz (continuous wave (CW)) at 37, 41, and 42.5°C.
| 周波数 | 2.45 GHz |
|---|---|
| ばく露時間 | 30 min |
| Modulation type | CW |
|---|
| ばく露の発生源／構造 | |
|---|---|
| ばく露装置の詳細 | Test tubes containing spleen cells were placed vertically in a microwave-absorber-line chamber 45 cm below the aperture of antenna (15 x 20 cm) |
| Additional information | Cells were heated at 37, 41 and 42.5°C |
The data show that for the nonirradiated controls, capping is reduced from 90% at 37°C, to 52% at 41°C, to less than 5% for cells that were pretreated at 42.5°C. There was no significant difference between the exposed cells and the control cells when both were maintained at the same temperature. In another experiment (field 2), there was no significant difference in the percentage of capping between control cells and cells that were exposed to microwave irradiation during capping, when the temperature in both preparations was kept at 38.5°C.
The data indicate that the mechanism responsible for inhibition of capping are thermal in origin. Further studies are needed to examine what cellular components are affected by heat, resulting in inhibition of capping, and what would be the physiological significance of such inhibition at temperatures often associated with fever and hyperthermia.
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