研究のタイプ: 医学／生物学の研究 (experimental study)

[マイクロ波放射されたセルトリ細胞で産生されたサイトカインはラット精巣での精子形成を妨害する] med./bio.

Cytokines produced by microwave-radiated Sertoli cells interfere with spermatogenesis in rat testis

著者: Wu H, Wang D, Shu Z, Zhou H, Zuo H, Wang S, Li Y, Xu X, Li N, Peng R
掲載誌: Andrologia 2012; 44: 590-599
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この研究は、マイクロ波ばく露を受けたラットのセルトリ細胞におけるサイトカイン産生を評価し、さらに、マイクロ波ばく露を受けたセルトリ細胞と生殖細胞の共培養が生殖細胞のアポトーシスおよび酸化ストレスに及ぼす影響を調べた。特にTNFalpha、IL-1beta、IL-6が生殖細胞にどのように影響するかに着目した。その結果、マイクロ波ばく露を受けたセルトリ細胞では、TNFalpha、IL-1betaおよびIL-6の増加が示された；これら増加したサイトカインは、生殖細胞の膜にアポトーシスと脂質過酸化を誘発する可能性がある；さらに、生殖細胞のアポトーシスは、Bax / Bcl-2およびカスパーゼ-3の発現増加に関連していた、と報告している。

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研究目的（著者による）

To examine the synthesis of cytokines in microwave exposed Sertoli cells of rats and to investigate the effect of a co-incubation of microwave exposed Sertoli cells with germ cells on apoptosis and oxidative stress in germ cells.

詳細情報

Spermatogenesis is the process of male germ cell development from the primordial germ cells to the mature sperm cell. This process can be disturbed by several factors. In this study, molecular mechanisms potentially influencing the spermatogenesis should be examined.
Sertoli cells were isolated from 3-week-old male rats and exposed to microwaves or sham exposed. Afterwards, the synthesis of cytokines was determined. Additionally, the exposed/sham exposed Sertoli cells were re-suspended with trypsin and co-incubated with germ cells (isolated from 8-week-old male rats) in a cell culture. After 24 hours of incubation, the germ cells were collected for further analysis (apoptosis, oxidative stress).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 0 Hz
ばく露時間: continuous for up to 24 hours
S Band microwaves

ばく露1

主たる特性
周波数 0 Hz
タイプ
  • electromagnetic field
ばく露時間 continuous for up to 24 hours
Additional information S Band microwaves
ばく露装置
ばく露の発生源／構造
  • 詳細不明
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
電力密度 100 mW/cm² mean - - -

Reference articles

  • Zuo HY et al. (2009): [ラット海馬のRAF / MEK / ERKシグナル伝達経路への電磁放射の影響]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

Sertoli cells: The gene expression of the tumor necrosis factor-alpha was significantly increased after 2 hours of exposure compared to the sham exposure, while the gene expression of interleukin-1beta and interleukin-6 was significantly increased after 2 and 6 hours of exposure. In the culture medium, the levels of tumor necrosis factor-alpha, interleukin-1beta and interleukin-6 were significantly increased after 24 hours of exposure in comparison to sham exposed cell cultures.
Germ cells: In the culture medium of germ cells which were co-incubated with exposed Sertoli cells, lipid peroxidation was significantly increased compared to germ cells co-incubated with sham exposed Sertoli cells. Additionally, co-incubation of germ cells with exposed Sertoli cells, led to a significantly increased apoptosis rate in germ cells in comparison to germ cells co-incubated with sham exposed Sertoli cells.
The authors suggest that exposure of rat Sertoli cells could have increased the synthesis of cytokines and that these cytokines could have an influence on the spermatogenesis in rats.

研究の種別:

研究助成

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